PMID: 4289964Jun 1, 1966Paper

Studies on the function of intracellular ribonucleases. V. Ribonuclease activity in ribosomes and polysomes prepared from rat liver and hepatomas

The Journal of Cell Biology
T Utsunomiya, J S Roth

Abstract

The RNase activity and properties of ribosome and polysome preparations from normal rat liver and some hepatomas have been examined. Polysome and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hepatomas had considerably higher specific RNase activity than corresponding preparations from normal rat liver, Novikoff ascites, or Morris 5123 hepatomas. The optimum pH of the RNase was approximately 8.5 for all samples tested, and the samples showed no evidence of latent RNase activity when treated with 3 M sodium chloride, EDTA, urea, or p-chloromercuribenzenesulfonic acid. The RNase activity appeared to be associated principally with breakdown products and/or subunits smaller than 80S. In the presence of Mg(++) ions, subunits could reaggregate to form monomer ribosomes indistinguishable from the natural products, but some of the reassociated ribosomes could contain RNase activity which had been bound to the smaller particles. Similar results were obtained with spermine. In the hepatomas, evidence was obtained for the preexistence of considerable amounts of the smaller, RNase-containing subunits in the cell. When a small amount of crystalline bovine pancreatic RNase was added to partly dissociated ribosomes, the RNa...Continue Reading

References

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Citations

Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J M García-Segura, J G Gavilanes
Sep 15, 1972·FEBS Letters·O C. IngebretsenA Pihl
Jan 1, 1970·Archives of Biochemistry and Biophysics·A J Dunn
Jul 1, 1975·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P Eker
May 13, 1971·Biochimica Et Biophysica Acta·C O Enwonwu, H N Munro
Jan 1, 1967·Biochimica Et Biophysica Acta·J R Smeaton, W H Elliott
Apr 24, 1968·Biochimica Et Biophysica Acta·C Quirin-StrickerP Mandel
Jul 18, 1967·Biochimica Et Biophysica Acta·J Minguell, M Perretta

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