PMID: 6986611Jan 11, 1980Paper

Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction

Nucleic Acids Research
B PoldermansP H van Knippenberg

Abstract

The effect of the presence or absence of the methylgroups of the m2(6)Am2(6)A sequence near the 3' end of 16S rRNA of Escherichia coli on the interaction of the ribosomal subunits has been studied, using wild-type (methylated) and mutant (unmethylated) ribosomes. Subunit exchange experiments and competitive association experiments show a strong preference of the 50S subunit for association with methylated 30S subunits. The results indicate that the equilibrium constant of the reaction 70S in equilibrium with 30S + 50S is dependent on the methylgroups; mutant 30S.50S couples are less stable than wild-type 30S.50S couples. It is postulated that the methylgroups also stimulate the interaction between 30S subunits and initiation factor IF-3.

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Citations

Oct 1, 1996·Journal of Molecular Evolution·M S Springer, E Douzery
Jan 17, 2013·Nature Communications·Hasan DemirciGerwald Jogl
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chao TuXinhua Ji
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W F JacobA E Dahlberg
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D NègreJ Ofengand
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·P H van Knippenberg, H A Heus
Feb 25, 1982·Nucleic Acids Research·R Van Charldorp, P H Van Knippenberg
Oct 10, 1981·Nucleic Acids Research·R Van CharldorpP H Van Knippenberg
Jul 11, 2014·Molecular Biology of the Cell·Joanna RorbachMichal Minczuk
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Attilio FabbrettiPaola Fucini
Aug 5, 2014·Cell Metabolism·B Martin Hällberg, Nils-Göran Larsson
May 31, 2012·The Biochemical Journal·Joanna Rorbach, Michal Minczuk
Jul 10, 1998·Structure·J P Rife, P B Moore
Dec 26, 2006·Molecular Cell·Nicholas D BonawitzGerald S Shadel
Apr 7, 1998·Molecular and Cellular Biology·D L LafontaineD Tollervey
May 18, 2017·The Biochemical Journal·Agata RozanskaRobert N Lightowlers
Jan 13, 2021·Nucleic Acids Research·Robert KnüppelSébastien Ferreira-Cerca

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