Studies on the hydrolysis of 1-alkyl-sn-glycero-3-phosphoethanolamine in subcellular fractions of rat brain

Hoppe-Seyler's Zeitschrift für physiologische Chemie
M VierbuchenH Debuch

Abstract

The formation of 1-alkylglycerol from 1-alkyl-sn-glycero-3-phosphoethanolamine in different cell fractions of rat brain is reported. The substrates used were labelled either with 14C or 3H in the alkyl residue or with 14C in the alkyl and 3H in the ethanolamine residue. The examination of the lipid- and water-soluble cleavage products showed that both ethanolamine and phosphoethanolamine are liberated from the substrate in the microsomal fraction of 14-day-old rat brain. The latter product is rapidly hydrolyzed. In comparison with other cell fractions, the microsomes contained the highest enzyme activities, which exhibited a pH optimum of 7.1--7.5. SH-group reagents are inhibitors, whereas diisopropylfluorophosphate has no effect. As the animals age, these enzyme activities decrease in brain homogenates.

Citations

Jul 1, 1975·Archives of Biochemistry and Biophysics·M Saito, J Kanfer
Nov 1, 1977·Archives of Biochemistry and Biophysics·R L WykleJ M Schremmer
Mar 5, 1973·Biochemical and Biophysical Research Communications·K ChaeF Snyder
Jan 1, 1970·Biochimica Et Biophysica Acta·J G Parkes, W Thompson
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

Related Concepts

Microsomes
Isotope Labeling, Stable
Brain
Carbon Radioisotopes
Tritium
Subcellular Fractions
Phosphatidylethanolamines
Hydrolysis
Hydrogen-Ion Concentration
Metazoa

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