Studies on the immune response and preparation of antibodies against a large panel of conjugated neurotransmitters and biogenic amines: specific polyclonal antibody response and tolerance

Journal of Neurochemistry
Han HuismanPeter W Setter

Abstract

We described the production and characterization of antibodies against three important groups of neuro-active haptens, e.g., neurotransmitters and biogenic amines. First, from the tryptophane metabolic pathway: tryptamine, serotonin, 5-hydroxy-indole acetic acid, and melatonin. Secondly, the tyrosine metabolic pathway: tyramine, dopamine, dihydroxyphenyl acetic acid, and norepinephrine. Thirdly, antibodies against excitatory and inhibitory neurotransmitters: glycine, glutamate, glutamine, and GABA. Immunogenic conjugates were prepared after linking haptens to carrier proteins. Most antibodies displayed high specificity against corresponding neuro-active haptens conjugated in vitro and in situ in biological specimens, but not to closely related conjugated metabolites, precursors, pharmaceuticals, agonists, antagonists, or free neuro-active haptens. Conjugated norepinephrine was highly tolerant in different animal species and produced incidentally a short specific antibody response.

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Citations

Dec 14, 2011·Analytical and Bioanalytical Chemistry·Mikaela I NichkovaGottfried H Kellermann
Aug 11, 2010·Neuroscience and Biobehavioral Reviews·David T MarcKelly L Olson
Mar 28, 2013·Journal of Neurochemistry·Mikaela NichkovaGottfried H Kellermann
Dec 21, 2010·Child: Care, Health and Development·D R CrossH Huisman

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