PMID: 5919689Aug 1, 1966

Studies on the immunochemistry of streptococcal mucopeptide

The Journal of Experimental Medicine
W W Karakawa, R M Krause

Abstract

Streptococcal mucopeptide, solubilized by either ultrasonic treatment or lysozyme, gave a precipitin reaction with rabbit antimucopeptide serum. A haptenic inhibitor of this reaction, which was composed of alanine, glutamic acid, and lysine in a mole ratio of 4:1:1, was isolated from a Streptomyces albus enzymes digest of Group D cell walls by ion exchange chromatography. When selected antisera were employed, greater than 90% inhibition of the mucopeptide quantitative precipitin reaction was achieved with 2 mg/ml of this inhibitor, whereas a hexosamine fraction with minimal concentrations of amino acid residues was inactive in this respect. These results suggest that the peptide moiety is an antigenic determinant of mucopeptide. Preliminary results indicate that the hexosamine polymer of the mucopeptide is a secondary antigenic determinant.

References

Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D J Tipper, J L Strominger
Nov 1, 1965·The Journal of Experimental Medicine·J RottaR M Krause
Jul 25, 1959·Nature·J J ARMSTRONGF C NEUHAUS
Feb 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·E M ABDULLA, J H SCHWAB

Citations

Aug 1, 1968·The Journal of Experimental Medicine·W W KarakawaR M Krause
Jul 1, 1969·The Journal of Experimental Medicine·J Rotta, B Bednár
Nov 1, 1973·The Journal of Experimental Medicine·V A BokischR M Krause
Jan 1, 1979·The Journal of Experimental Medicine·J Y TaiR C Lancefield
Mar 1, 1971·European Journal of Biochemistry·R C HughesM R Salaman
Sep 18, 2004·American Journal of Reproductive Immunology : AJRI·Ida HoláskováRobert A Dailey
Jul 17, 2004·Microbiology·David G PritchardJeffrey A Engler
Aug 1, 1973·Acta Pathologica Et Microbiologica Scandinavica. Section B: Microbiology and Immunology·S HelgelandK H Schleifer
Mar 14, 1973·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·C P SodomannO Haferkamp

Related Concepts

Antigen-Antibody Reactions
Antigens
Molecular Sieve Chromatography
Ion-Exchange Chromatography Procedure
Polypeptides
Precipitin Tests
Streptococcus

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