PMID: 6159462Nov 1, 1980Paper

Studies on the influence of tryptophan and related compounds on hepatic polyribosomes and protein synthesis in the rat

The Journal of Nutrition
H SidranskyC N Murty

Abstract

The effect of the administration of L-tryptophan or tryptophan-related compounds on rat liver RNA and protein metabolism was investigated. The five biochemical parameters studied were polyribosomal aggregation, protein synthesis in vitro, synthesis of cytoplasmic poly(A)-mRNA, release of labeled nuclear RNA in vitro (nuclear and cell sap effects) and nuclear envelope nucleoside triphosphatase activity. The administration of L-tryptophan to overnight fasted rats revealed a rapid stimulation in all of the five parameters. Also, some of the tryptophan-related compounds, especially 5-hydroxy-DL-tryptophan, 3-indolepyruvic acid, indole or 3-hydroxyanthranilic acid, revealed stimulation in many of the parameters. However, when rats were pretreated with puromycin to inhibit protein synthesis, only the administration of L-tryptophan was still able to stimulate significantly the hepatic polyribosomes, in vitro protein synthesis, in vitro nuclear RNA release (involving nuclei but not cell sap) and nuclear envelope nucleoside triphosphatase activity. The effects by the other tryptophan-related compounds became inhibited. The results emphasize the unique action of L-tryptophan on hepatic protein synthesis.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.