PMID: 9557877Apr 29, 1998Paper

Studies on the interaction between hyaluronan and a rat colon cancer cell line

Glycoconjugate Journal
C Samuelsson, S Gustafson

Abstract

Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.

Citations

Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Dan Peer, Rimona Margalit
Mar 16, 2004·The Journal of Biological Chemistry·Borhane AnnabiRichard Béliveau
Nov 2, 2005·Inflammopharmacology·C A S AlamD A Willoughby
Jun 21, 2007·Upsala Journal of Medical Sciences·Torvard C Laurent
Dec 19, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Anekant Jain, Sanjay K Jain
May 7, 2021·Biochemical and Biophysical Research Communications·Keita YamanaAtsushi Ikeda
Nov 17, 2001·Biomacromolecules·Y LuoG D Prestwich
Oct 1, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Anekant Jain, Sanjay K Jain
Sep 30, 1999·Bioconjugate Chemistry·Y Luo, G D Prestwich
May 19, 2009·Nanomedicine : Nanotechnology, Biology, and Medicine·Anekant JainAadil M Beg
Jul 6, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Henry J GreynerMark E Mummert

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