PMID: 3755060Jul 10, 1986Paper

Studies on the interaction of anesthetic steroids with phosphatidylcholine using 2H and 13C solid state NMR

Biochimica Et Biophysica Acta
A MakriyannisR G Griffin

Abstract

The effects of the anesthetic steroid alphaxalone and its inactive analog delta 16-alphaxalone on model phospholipid membranes were studied using 13C and 2H solid-state nuclear magnetic resonance spectroscopy. Aqueous multilamellar dispersions of dipalmitoylphosphatidylcholine (DPPC) with specific 13C and 2H labels as endogenous probes at the carbonyl and the C-7 methylene groups, respectively, of the sn-2 chain were used to study the conformational and dynamical properties of the bilayer as a function of temperature. There were no significant changes between the 13C and 2H spectra of the DPPC preparation containing the inactive steroid and that of DPPC with no drug. However, the physiologically active steroid produces significant spectral 2H and 13C changes. These changes include a reduction of the main phase transition temperature and a broadening of that transition. Alphaxalone also increases the relative number of gauche conformers in the liquid-crystalline phase of DPPC and increases the rate of axial diffusion in both the gel and liquid-crystalline phase. The thermotropic properties of the above preparations, as monitored by differential scanning calorimetry, were congruent with the spectroscopic data.

Citations

Feb 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A Sanchez-BuenoT Saito
Apr 8, 1999·Chemistry and Physics of Lipids·R A VideiraV M Madeira
Jan 27, 2015·Biochimica Et Biophysica Acta·Mattia SacchiAndrea Alessandrini
Sep 2, 2006·Biochimica Et Biophysica Acta·Juha-Matti AlakoskelaPaavo K J Kinnunen
Feb 11, 2015·Biochimica Et Biophysica Acta·Daniel BallezaAndrea Alessandrini

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