Studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2

Microbiology
M KesselerGerhard Gottschalk

Abstract

The enzymes responsible for the degradation of isopropylbenzene (IPB) and co-oxidation of trichloroethene (TCE) by Rhodococcus erythropolis BD2 are encoded by the linear plasmid pBD2. Fragments containing IPB catabolic genes were cloned from pBD2 and the nucleotide sequence was determined. By means of database searches and expression of the cloned genes in recombinant strains, we identified five clustered genes, ipbA1A2A3A4C, which encode the three components of the IPB 2,3-dioxygenase system, reductaseIPB (ipbA4), ferredoxinIPB (ipbA3) and the two subunits of the terminal dioxygenase (ipbA1A2), as well as the 3-isopropylcatechol (IPC) 2,3-dioxygenase (ipbC). The protein sequences deduced from the ipbA1A2A3A4C gene cluster exhibited significant homology with the corresponding proteins of analogous degradative pathways in Gram-negative and Gram-positive bacteria, but the gene order differed from most of them. IPB 2,3-dioxygenase and 3-IPC 2,3-dioxygenase could both be expressed in Escherichia coli, but the IPB 2,3-dioxygenase activities were too low to be detected by polarographic and TCE degradative means. However, inhibitor studies with the R. erythropolis BD2 wild-type are in accordance with the involvement of the IPB 2,3-dio...Continue Reading

Citations

Apr 8, 2004·Applied Microbiology and Biotechnology·Horst PriefertAnthony J Sinskey
Jun 29, 2005·Applied Microbiology and Biotechnology·Shin-Ichi HiranoShigenori Kanaya
Sep 6, 2000·Journal of Applied Microbiology·A Vomberg, U Klinner
Mar 4, 2000·Applied and Environmental Microbiology·M M BreslerN C Bruce
Jun 6, 2003·Applied and Environmental Microbiology·Brett R BaldwinLoring Nies
Oct 7, 2004·Applied and Environmental Microbiology·Anthony S DankoDavid L Freedman
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kyung-Su NaJunichi Kato
Apr 10, 2013·Journal of Bioscience and Bioengineering·Seikoh SaitohToru Matsui
Aug 7, 2002·Biochemical and Biophysical Research Communications·Hideaki NojiriToshio Omori
Sep 29, 2004·FEMS Microbiology Reviews·Volker GürtlerRobert Seviour
Jun 16, 2015·Frontiers in Microbiology·Julián R DibFriedhelm Meinhardt
Jan 12, 2012·PloS One·Michelle Christiane da Silva RabelloSylvia Cardoso Leão
Jun 29, 2002·Applied and Environmental Microbiology·Sandra TrottAndreas Stolz
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Seung-Bong LeeRussell P Herwig
May 3, 2019·BMC Genomics·Govinda GuevaraJuana María Navarro-Llorens
Jun 24, 1998·Biochemical and Biophysical Research Communications·J M Shepherd, G Lloyd-Jones

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.