Abstract
1. A mutant (ANT 8) of Schizosaccharomyces pombe which shows resistance to antimycin both in vivo and in vitro is characterized biochemically and genetically. 2. In crosses of ANT 8 with auxotrophic strains, resistance to antimycin segregates 2:2 indicating that resistance is conferred by a single nuclear gene. Diploids heterozygous for the resistance gene, however, show segregation of the resistance and sensitivity during mitosis. Possible reasons for this segregation are discussed. 3. Compared with the wild type, the NADH oxidase of ANT 8 requires 13 times as much antimycin for 95% inhibition. After addition of ubiquinone-3, electron transport which is less sensitive to antimycin is found only in the mutant. 4. The resistance of the mutant ANT 8 si due to the much weaker binding of antimycin to mitochondria. As in the wild type, two antimycin binding sites can be separated by binding studies. From the inhibition curves it is evident that binding of antimycin to oxidized mitochondrial particles does not correspond with its inhibitory effect on the partly reduced enzyme in kinetic studies. 5. The peak of the b-cytochrome absorbing at 560.2 nm at 77 degrees K in the wild type is shifted to 561 nm in the mutant. 6. A special prep...Continue Reading
References
Apr 27, 1973·Biochimica Et Biophysica Acta·C J Grimmelikhuijzen, E C Slater
Jan 1, 1971·Molecular & General Genetics : MGG·K WolfF Kaudewitz
Sep 1, 1972·Biochimica Et Biophysica Acta·W Bandlow
Oct 18, 1974·Biochimica Et Biophysica Acta·B LangW Bandlow
Oct 1, 1970·Journal of Bacteriology·H HeslotC Louis
Feb 28, 1972·Biochimica Et Biophysica Acta·J A Berden, E C Slater
Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A W LinnaneH B Lukins
Feb 1, 1970·Mutation Research·P Munz, U Leupold
Oct 1, 1970·Journal of Bacteriology·H HeslotA Goffeau
Oct 2, 1970·Genetical Research·M Flores da Cunha
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX
Citations
Oct 24, 1977·Molecular & General Genetics : MGG·G SeitzF Kaudewitz
Jan 1, 1987·Current Genetics·A BrunnerA Tuena de Cobos
Sep 1, 1984·Current Genetics·F AhneK Wolf
Mar 7, 1977·Molecular & General Genetics : MGG·D J ObbinkA W Linnane
Sep 12, 1988·FEBS Letters·S Weber, K Wolf
Jul 1, 1983·Current Genetics·P Q AnzianoK Wolf
Jan 1, 1977·Protoplasma·L W Olson, U M Edén
Jul 23, 1976·Molecular & General Genetics : MGG·G Michaelis
Apr 29, 1977·Molecular & General Genetics : MGG·L Del GiudiceF Kaudewitz
May 1, 1980·European Journal of Biochemistry·G Burger
Sep 8, 1978·Molecular & General Genetics : MGG·G Seitz-MayrF Kaudewitz
Jan 1, 1980·Pharmacology & Therapeutics·J S Rieske
Sep 27, 1976·Biochimica Et Biophysica Acta·J S Rieske
Jan 1, 1987·Biochimie·P Chevillotte-BrivetD Meunier-Lemesle
Aug 11, 1975·Biochimica Et Biophysica Acta·G BurgerF Kaudewitz
Jan 1, 1977·Analytical Biochemistry·B LangF Kaudewitz
Oct 4, 1976·Biochemical and Biophysical Research Communications·G BurgerF Kaudewitz