PMID: 5920194Apr 1, 1966

Studies on the mechanism of hormonal induction of alkaline phosphatase in human cell cultures. I. Effects of puromycin and actinomycin D

The Journal of Cell Biology
M J Griffin, R P Cox

Abstract

Increased alkaline phosphatase activity is induced in certain epithelial cell cultures by hormones with adrenal glucocorticoid activity or their analogues such as prednisolone (Delta(I)-hydrocortisone). Enzyme induction occurs in two distinct phases. During the first 12 hr after the addition of prednisolone, there is a small increase in alkaline phosphatase levels. After 15 to 24 hr, the enzyme activity shows a sudden, marked linear rise, reaching a maximum at 60 to 80 hr. Puromycin blocks enzyme induction immediately, even when added during the period of rapid increase of enzyme. Actinomycin D blocks induction when added no later than 8 hr after the addition of prednisolone. On the other hand, Actinomycin D added during the phase of rapid enzyme induction has no effect for at least 12 hr. These findings suggest that de novo protein synthesis is involved in prednisolone induction of alkaline phosphatase and that the RNA messenger for this enzyme is relatively stable.

References

Jan 1, 1962·The Journal of General Physiology·R P Cox, C M MACLEOD
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·R M FRANKLIN, D BALTIMORE
Apr 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R P Cox, C M MACLEOD
Nov 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·H L SEGAL, Y S KIM
Jan 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·C ARPELSC M SOUTHAM
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·M REVEL, H H HIATT
Jan 1, 1964·Cold Spring Harbor Symposia on Quantitative Biology·R P Cox, C M MACLEOD
Apr 23, 1965·Biochemical and Biophysical Research Communications·S BLOOMH GREEN

Citations

Dec 1, 1969·Biochemical Genetics·N A Elson, R P Cox
Mar 1, 1991·Biochemical Pharmacology·P V Taberner, D M Connelly
Mar 1, 1967·European Journal of Pharmacology·O A Olsson
Feb 18, 1971·The New England Journal of Medicine·B W O'Malley
Apr 1, 1978·British Journal of Haematology·R D MacaJ C Hoak
Oct 14, 1969·Annals of the New York Academy of Sciences·M J Griffin, R H Bottomley
May 1, 1969·Transactions of the New York Academy of Sciences·B W O'Malley
Aug 1, 1967·The Journal of Experimental Zoology·K V Prahlad, L E DeLanney
Jun 1, 1969·Archives of Biochemistry and Biophysics·M J Griffin
Oct 1, 1967·Experimental Cell Research·E Eliasson
Sep 1, 1967·Archives of Biochemistry and Biophysics·S Karpatkin, A Samuels
May 1, 1974·The American Journal of Medicine·W H Fishman
Feb 1, 1974·Metabolism: Clinical and Experimental·E B Thompson, M E Lippman
Dec 1, 1967·Connecticut Medicine·R N Barnett
Dec 1, 1972·Biochimica Et Biophysica Acta·S H TuM J Griffin
Nov 1, 1977·Biochimica Et Biophysica Acta·B SeetharamD H Alpers

Related Concepts

Cell Division Phases
Ac-De
HeLa Cells
Leucine
Predonine
Puromycin Dihydrochloride
Poly(A) Tail
Culture Techniques

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.