PMID: 5920194Apr 1, 1966

Studies on the mechanism of hormonal induction of alkaline phosphatase in human cell cultures. I. Effects of puromycin and actinomycin D

The Journal of Cell Biology
M J Griffin, R P Cox


Increased alkaline phosphatase activity is induced in certain epithelial cell cultures by hormones with adrenal glucocorticoid activity or their analogues such as prednisolone (Delta(I)-hydrocortisone). Enzyme induction occurs in two distinct phases. During the first 12 hr after the addition of prednisolone, there is a small increase in alkaline phosphatase levels. After 15 to 24 hr, the enzyme activity shows a sudden, marked linear rise, reaching a maximum at 60 to 80 hr. Puromycin blocks enzyme induction immediately, even when added during the period of rapid increase of enzyme. Actinomycin D blocks induction when added no later than 8 hr after the addition of prednisolone. On the other hand, Actinomycin D added during the phase of rapid enzyme induction has no effect for at least 12 hr. These findings suggest that de novo protein synthesis is involved in prednisolone induction of alkaline phosphatase and that the RNA messenger for this enzyme is relatively stable.


Jan 1, 1962·The Journal of General Physiology·R P Cox, C M MACLEOD
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·R M FRANKLIN, D BALTIMORE
Apr 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R P Cox, C M MACLEOD
Nov 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·H L SEGAL, Y S KIM
Jan 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·C ARPELSC M SOUTHAM
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·M REVEL, H H HIATT
Jan 1, 1964·Cold Spring Harbor Symposia on Quantitative Biology·R P Cox, C M MACLEOD
Apr 23, 1965·Biochemical and Biophysical Research Communications·S BLOOMH GREEN


Dec 1, 1969·Biochemical Genetics·N A Elson, R P Cox
Mar 1, 1991·Biochemical Pharmacology·P V Taberner, D M Connelly
Mar 1, 1967·European Journal of Pharmacology·O A Olsson
Feb 18, 1971·The New England Journal of Medicine·B W O'Malley
Apr 1, 1978·British Journal of Haematology·R D MacaJ C Hoak
Oct 14, 1969·Annals of the New York Academy of Sciences·M J Griffin, R H Bottomley
May 1, 1969·Transactions of the New York Academy of Sciences·B W O'Malley
Aug 1, 1967·The Journal of Experimental Zoology·K V Prahlad, L E DeLanney
Jun 1, 1969·Archives of Biochemistry and Biophysics·M J Griffin
Oct 1, 1967·Experimental Cell Research·E Eliasson
Sep 1, 1967·Archives of Biochemistry and Biophysics·S Karpatkin, A Samuels
May 1, 1974·The American Journal of Medicine·W H Fishman
Feb 1, 1974·Metabolism: Clinical and Experimental·E B Thompson, M E Lippman
Dec 1, 1967·Connecticut Medicine·R N Barnett
Dec 1, 1972·Biochimica Et Biophysica Acta·S H TuM J Griffin
Nov 1, 1977·Biochimica Et Biophysica Acta·B SeetharamD H Alpers

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