Studies on the mechanism of inhibition of amphibian oocyte adenylate cyclase by progesterone

Archives of Biochemistry and Biophysics
X JordanaJ E Allende

Abstract

Progesterone treatment induces the meiotic maturation of Xenopus laevis oocytes. Previous evidence indicates that this hormonal effect may be due to inhibition of oocyte adenylate cyclase. The present work studies several aspects of the mechanism of adenylate cyclase inhibition by this hormone. Forskolin greatly stimulates oocyte adenylate cyclase in the absence of guanine nucleotides and this activity is not sensitive to progesterone inhibition. In addition the forskolin-activated enzyme is not inhibited by a wide range of guanine nucleotide, in the presence or absence of hormone. The time course of cAMP synthesis catalyzed by oocyte adenylate cyclase in the presence of guanyl-5'l-imidodiphosphate (Gpp(NH)p) shows an initial lag period that does not depend on the concentration of Gpp(NH)p. Progesterone causes a very significant increase in the hysteresis of the reaction, at least doubling the half-time of enzyme activation. The hormonal effect on the lag cannot be reversed by saturating concentrations of Gpp(NH)p. Progesterone also decreases the steady-state rates of the reaction. This effect, however, depends on the concentration of Gpp(NH)p. High concentrations of Gpp(NH)p almost completely reverse the inhibition of the stea...Continue Reading

References

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Citations

Sep 1, 1986·Fish Physiology and Biochemistry·H R Habibi, C A Lessman
Jun 1, 1985·Cell Differentiation·J L Maller
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D CarrascoJ E Allende
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M AntonelliJ E Allende
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G JacobJ E Allende
Dec 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G JacobJ E Allende
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M GaticaJ E Allende
Sep 5, 2002·Molecular Reproduction and Development·Ximena RomoJuan Olate
Sep 25, 2004·Journal of Cellular Physiology·Leonardo GuzmánJuan Olate
Oct 26, 2007·Journal of Cellular Physiology·Ximena RomoJuan Olate
Dec 23, 2006·Molecular Endocrinology·Liat Josefsberg Ben-YehoshuaJames L Maller
Jul 31, 2002·Archives of Biochemistry and Biophysics·Murat OzCharles E Spivak

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