Studies on the mechanism of luteinizing hormone-induced desensitization of the rabbit follicular adenylyl cyclase system in vitro

Endocrinology
M Hunzicker-Dunn, L Birnbaumer

Abstract

In vitro studies were conducted to evaluate the possible mechanisms of LH-induced desensitization of the rabbit follicular adenylyl cyclase (AC) system. We tested the effects of cAMP, dibutyryl cAMP, and inhibitors of various cellular functions on LH-stimulated AC activity as well as the reversibility of AC desensitization. Refractoriness of the AC to LH was induced by a1 1- or 2-h incubation of Graafian follicles with 10 microgram/ml LH. We fund that the initial 60-min phase of AC desensitization to LH in Graafian follicles was not prevented by a 60-min preincubation of follicles with 11 microM puromycin, 30 microM cycloheximide, 8 microM actinomycin D, 5 microgram/ml cytochalasin B, 50 microM colchicine, or 1 or 10 mM trinitrophenol or by a 90-min preincubation of follicles with 50 microM colchicine. We evaluated the effects of cAMP and dibutyryl cAMP on LH-stimulated AC activity by incubating Graafian follicles with these nucleotides for 30-min to 4 h. While LH-stimulated AC activity was not significantly reduced in follicles which had been incubated 30-min or 1-h with either nucleotide, 2 h incubations resulted in significant reductions in LH-stimulated AC activity, and 4-h incubations promoted a complete refractoriness of ...Continue Reading

Citations

Mar 1, 1983·Molecular and Cellular Endocrinology·L Hedin, K Ahrén
Jan 1, 1984·Molecular and Cellular Endocrinology·H AttramadalV Hansson
Nov 1, 1987·Molecular and Cellular Endocrinology·L T Budnik, A K Mukhopadhyay
Aug 2, 2003·Biology of Reproduction·Deborah A Roess, Steven M L Smith
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S MukherjeeM Hunzicker-Dunn

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