PMID: 8596718Mar 1, 1996Paper

Studies on the mechanism of swelling-induced lysosomal alkalinization in vascular smooth muscle cells

Pflügers Archiv : European journal of physiology
G L BuschF Lang

Abstract

Previous studies in renal cells and hepatocytes have shown that cell swelling leads to a rapid and reversible increase in pH in acidic cellular compartments, including lysosomes. Among the consequences are an inhibition of proteolysis. The present study shows that a similar lysosomal alkalinization occurs upon osmotic swelling of vascular smooth muscle cells, as evidenced by acridine orange and fluorescein isothiocyanate fluorescence. Furthermore, we have studied the mechanism underlying lysosomal alkalinization, which had remained unclear. The lysosomal alkalinization was not abolished by inhibition of vacuolar H+-ATPases (100 nM bafilomycin), Cl- channels [100 microM] 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), carbonic anhydrase (100 microM acetazolamide) or Na+/H+ exchange (10 microM HOE 694). The Ca2+ ionophore A23187 (10 microM) led to a slight increase in lysosomal pH, but removal of extracellular Ca2+ and depletion of cellular Ca2+ stores (100 nM thapsigargin) did not appreciably blunt the swelling-induced lysosomal alkalinization. In the presence of bafilomycin the alkalinizing effect of osmotic cell swelling was not reversible, in contrast to that of NH4Cl. In conclusion, osmotic swelling of vascular smooth m...Continue Reading

References

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Citations

Jan 27, 2009·Molecular Genetics and Metabolism·Shoichi TakikitaPaul H Plotz
Nov 23, 2006·The Biochemical Journal·Anthony J Morgan, Antony Galione
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Jan 24, 2003·American Journal of Physiology. Cell Physiology·S McGinnC A Pollock
Oct 26, 2007·The Journal of Biological Chemistry·Anthony J Morgan, Antony Galione
Nov 14, 1997·The American Journal of Physiology·A Krämer-GuthF Lang
Mar 14, 2003·Biochimica Et Biophysica Acta·Reinout P HesselinkGer J Van der Vusse
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Markus RitterHubert H Kerschbaum

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