PMID: 6405798May 25, 1983Paper

Studies on the mechanisms by which 2-chloroadenosine stimulates bone resorption in tissue culture

Biochimica Et Biophysica Acta
U H Lerner, B B Fredholm

Abstract

The effect of 2-chloroadenosine on bone resorption was studied in calvarial bones from 6-7-day-old mice in organ culture. 2-Chloroadenosine stimulated the mobilization of minerals (40Ca, 45Ca) and increased the degradation of matrix ([3H]proline) from the bones. The nucleoside also caused an increased release of beta-glucuronidase, a lysosomal enzyme. In doses above 30 microM 2-chloroadenosine was cytotoxic, as evidenced by an increased release of lactate dehydrogenase. 2-Chloroadenosine-stimulated resorption could be inhibited by calcitonin, increased concentration of phosphate in culture medium, cortisone, dexamethasone, indomethacin, naproxen, meclofenamic acid and 5,8,11,14-eicosatetraynoic acid. 2-Chloroadenosine was much more sensitive to inhibition by dexamethasone than was parathyroid hormone. The response to the maximal dose of 2-chloroadenosine could not be enhanced by parathyroid hormone, 1 alpha-hydroxyvitamin D-3 and prostaglandin E2. An exposure to 2-chloroadenosine for 12 h was not sufficient to produce prolonged resorption. The results suggest that 2-chloroadenosine stimulated bone resorption by a process which is dependent on osteoclastic activity. The possibility that the effect of 2-chloroadenosine, either di...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·I H Fox, W N Kelley
Dec 14, 1978·Biochemical and Biophysical Research Communications·A H Tashjian, L Levine
Aug 1, 1975·The Journal of Clinical Investigation·L G RaiszC L Trummel
Jun 1, 1981·Acta Endocrinologica·U Lerner, G T Gustafson
Jun 1, 1982·Acta Endocrinologica·U Lerner, B B Fredholm
Aug 1, 1981·The Journal of Experimental Medicine·R S Bockman, M A Repo
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

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Citations

Jun 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·O LjunggrenU H Lerner
Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Apr 1, 1984·Acta Physiologica Scandinavica·B B Fredholm, U Lerner
May 17, 2015·Autonomic Neuroscience : Basic & Clinical·Isabel R Orriss
Oct 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·U H Lerner

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