Abstract
Studies on the metabolism and on the toxicological analysis of mefenorex [R,S-N-(3-chloropropyl)-alpha-methylphenethylamine, MF] using gas chromatography-mass spectrometry (GC-MS) and fluorescence polarization immunoassay (FPIA) are described. The metabolites were identified in urine samples of volunteers by GC-MS. Besides MF, thirteen metabolites including amphetamine (AM) could be identified and three partially overlapping metabolic pathways could be postulated. For GC-MS detection, the systematic toxicological analysis procedure including acid hydrolysis, extraction at pH 8-9 and acetylation was suitable (detection limits 50 ng/ml for MF and 100 ng/ml for AM). Excretion studies showed, that only AM but neither MF nor its specific metabolites were detectable between 32 and 68 h after ingestion of 80 mg of MF. Therefore, misinterpretation can occur. The Abbott TDx FPIA amphetamine/methamphetamine II gave positive results up to 68 h. All the positive immunoassay results could be confirmed by the described GC-MS procedure.
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Citations
Dec 4, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roland F Staack, Hans H Maurer
Apr 15, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roland F Staack, Hans H Maurer
Jan 25, 2003·Journal of Biochemical and Biophysical Methods·Ju-Tsung Liu, Ray H Liu
Mar 18, 2000·Toxicology Letters·H H MaurerF T Peters
May 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roland F StaackHans H Maurer
Feb 12, 2004·Clinical Pharmacokinetics·Rafael de la TorreSimona Pichini
Jan 12, 2005·Journal of Mass Spectrometry : JMS·Denis S TheobaldHans H Maurer
Sep 25, 2003·Journal of Mass Spectrometry : JMS·Roland F StaackHans H Maurer
Mar 11, 2000·Drug Metabolism Reviews·F Musshoff
May 25, 2002·Journal of Occupational and Environmental Medicine·John T Cody
Jul 17, 2004·Therapeutic Drug Monitoring·Roland F StaackHans H Maurer
May 15, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keri A BarksdaleGautam N Bijur
Mar 19, 2002·Therapeutic Drug Monitoring·Thomas Kraemer, Hans H Maurer
Apr 8, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·H H MaurerT Kraemer