Studies on the metabolism and toxicological detection of the amphetamine-like anorectic mefenorex in human urine by gas chromatography-mass spectrometry and fluorescence polarization immunoassay

Journal of Chromatography. B, Biomedical Sciences and Applications
T KraemerH H Maurer

Abstract

Studies on the metabolism and on the toxicological analysis of mefenorex [R,S-N-(3-chloropropyl)-alpha-methylphenethylamine, MF] using gas chromatography-mass spectrometry (GC-MS) and fluorescence polarization immunoassay (FPIA) are described. The metabolites were identified in urine samples of volunteers by GC-MS. Besides MF, thirteen metabolites including amphetamine (AM) could be identified and three partially overlapping metabolic pathways could be postulated. For GC-MS detection, the systematic toxicological analysis procedure including acid hydrolysis, extraction at pH 8-9 and acetylation was suitable (detection limits 50 ng/ml for MF and 100 ng/ml for AM). Excretion studies showed, that only AM but neither MF nor its specific metabolites were detectable between 32 and 68 h after ingestion of 80 mg of MF. Therefore, misinterpretation can occur. The Abbott TDx FPIA amphetamine/methamphetamine II gave positive results up to 68 h. All the positive immunoassay results could be confirmed by the described GC-MS procedure.

References

Nov 1, 1975·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R G MooreJ Vine

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Citations

Apr 15, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roland F Staack, Hans H Maurer
Jan 25, 2003·Journal of Biochemical and Biophysical Methods·Ju-Tsung Liu, Ray H Liu
May 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roland F StaackHans H Maurer
May 25, 2002·Journal of Occupational and Environmental Medicine·John T Cody
May 15, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keri A BarksdaleGautam N Bijur

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