Studies on the Methyl Green-DNA complex and its dissociation by drugs.

Biochemistry
A K Krey, F E Hahn

Abstract

Spectrophotometric results indicated that Methyl Green bound stably to native calf thymus DNA and to poly[d(A-T)] but to a lesser extent to phiX 174 DNA, tRNAs, and poly(dG-dC), a copolymer that exists preferentially in the A conformation. Exposing the Methyl Green-DNA complex to graded concentrations of ethyl alcohol liberated part of the dye slowly by a zero-order reaction; higher alcohol concentrations which cause the B leads to A transition of DNA released the bulk of Methyl Green. The viscosity of the Methyl Green-DNA complex was significantly lower than that of the uncomplexed DNA. The dye was progressively liberated from DNA by 1.5 x 10(-1) M NaCl and by much lower concentrations of Mg2+; in its stoichiometric complex with DNA, it increased Tm by approximately 12 degrees C. A series of DNA-complexing drugs displaced Methyl Green from DNA at exponential rates and to end points which were correlated. End points of displacement correlated with the abilities of drugs to unwind supercoiled DNA, to labilize ribosomes to heat, and to eliminate a kanamycin resistance determinant from an R factor carried by Salmonella typhimurium. Additional correlations between Methyl Green displacement and biochemical-biological activities of d...Continue Reading

Citations

Dec 1, 1992·Journal of Molecular Recognition : JMR·C BaillyC Houssier
Jul 11, 1979·Nucleic Acids Research·R J FielN Datta Gupta
Mar 29, 2014·Histochemistry and Cell Biology·Daniel PrietoFlavio R Zolessi
Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A TatoJ M Ferrer
Mar 1, 1979·Analytical Biochemistry·D L Peters, M E Dahmus
Sep 27, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·P MurgaiN Varma

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