Studies on the methyl isocyanate adducts with globin

Chemico-biological Interactions
J MrázP Smigolová

Abstract

Isocyanates such as methylisocyanate (MIC), an intermediate in the synthesis of carbamate pesticides, or diisocyanates, used in the production of plastics, are highly reactive toxic compounds that spontaneously bind to biological macromolecules. In vivo formation of stable adducts with blood protein globin offers possibilities for biomonitoring of internal exposure to various reactive species. Thus, biomonitoring of the isocyanates through determination of their specific adducts with globin is a challenge. In this study, we characterized the adducts formed in human globin upon treatment with 100-fold molar excess of MIC. The globin was subject to enzymatic hydrolysis with pronase, and the hydrolysate was analysed by high performance liquid chromatography with positive atmospheric pressure chemical ionization mass spectrometric detection (HPLC/APCI-MS). The two major MIC adducts were those with N-terminal Val and side-chain of Lys, as confirmed by comparison with the synthetic standards. About 20 other adducts were observed, and several of them were tentatively identified using their MS and MS/MS spectra. Whereas detection of the adducts with Tyr and His was expected, the adducts with Trp and Phe, and a Lys adduct containing two...Continue Reading

References

Jan 15, 1990·Biochemical and Biophysical Research Communications·P G PearsonT A Baillie
Apr 29, 1988·Journal of Chromatography·P K RamachandranS Sriramachari
Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jaroslav MrázPetr Simek

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Citations

Nov 28, 2008·Journal of Occupational Medicine and Toxicology·Ervin C MingomatajEntela Hyso
Sep 28, 2012·Critical Reviews in Toxicology·Gerald L Kennedy
Apr 10, 2019·Polymers·Sylwia CzłonkaSaulius Vaitkus
Apr 2, 2020·Annals of the New York Academy of Sciences·Heidi J NickCarl W White
Jul 18, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Brian A LogueCarl W White
Jan 21, 2010·Chemical Research in Toxicology·Ronnie DaviesMargareta Törnqvist
Sep 18, 2007·Chemical Research in Toxicology·Michal JágrMartin Pospísil

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