PMID: 4294107Nov 1, 1967Paper

Studies on the mode of action of diphtheria toxin. 3. Site of toxin action in cell-free extracts

The Journal of Experimental Medicine
R S Goor, A M Pappenheimer

Abstract

Extracts from HeLa cells treated with excess diphtheria toxin for several hours, until all protein synthesis has been arrested, are still able to stimulate the poly U-directed incorporation of phenylalanine into polypeptides at a moderate rate. Activity may be restored to normal levels or above by addition of a soluble enzyme fraction containing transferase II. Our results are in agreement with those of Collier who has recently shown that toxin inactivates transferase II in extracts from rabbit reticulocytes. We have further demonstrated that amino acid incorporation in extracts from intoxicated HeLa cells is limited by their transferase II content whereas, in extracts from normal cells, it is the ribosomes and to a lesser extent sRNA that are limiting. We have found that only soluble transferase II is inactivated by toxin; the ribosome-bound enzyme is resistant.

References

Mar 1, 1967·The Journal of Experimental Medicine·J L Duncan, N B Groman
Feb 1, 1959·The Journal of Experimental Medicine·N STRAUSS, E D HENDEE
Jun 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·R ARLINGHAUSR SCHWEET
Dec 1, 1964·The Journal of Experimental Medicine·R J COLLIER, A M PAPPENHEIMER
Dec 1, 1964·The Journal of Experimental Medicine·R J COLLIER, A M PAPPENHEIMER

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Citations

Jan 1, 1978·Journal of Supramolecular Structure·M Vaughan, J Moss
Dec 1, 1973·Molecular Biology Reports·C A Van Der Mast, H P Bloemers
Nov 1, 1972·Die Naturwissenschaften·S Olsnes
Mar 16, 1968·Nature·R S Goor
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S RaeburnE S Maxwell
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J EverseN O Kaplan
Jun 1, 1968·The Journal of Experimental Medicine·A M Pappenheimer, R Brown
Dec 1, 1970·The Journal of Experimental Medicine·J B BasemanA A Harper
Feb 5, 2000·The Journal of Infectious Diseases·R K Holmes
May 1, 1994·Pediatric Pathology·N GoutasE Agapitos
Oct 10, 1968·Biochemical and Biophysical Research Communications·J A SchneiderE S Maxwell
May 1, 1978·FEBS Letters·D PuettJ P Robinson
Mar 23, 1974·FEBS Letters·D Vazquez
Mar 30, 1973·Nature·T R Ittelson, D M Gill
Feb 1, 1974·Archives internationales de physiologie et de biochimie·J Dirkx
Nov 1, 1977·CRC Critical Reviews in Toxicology·L J Berry
Jun 1, 1969·Journal of Bacteriology·J L Duncan, N B Groman
Dec 1, 1970·Bacteriological Reviews·L Barksdale
May 27, 1971·Biochimica Et Biophysica Acta·L MontanaroA Mattioli
Mar 1, 1975·Bacteriological Reviews·R J Collier
Sep 1, 1972·Archives of Biochemistry and Biophysics·D M Chuang, H Weissbach
Sep 30, 1970·Biochemical and Biophysical Research Communications·J A Traugh, R J Collier
Oct 26, 1973·Science·A M Pappenheimer, D M Gill
Jun 6, 1969·Science·R J Collier, H A Cole

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