PMID: 429208Feb 21, 1979Paper

Studies on the molecular arrangement of RNA in tissues with a selective topo-optical reaction of RNA

Histochemistry
J Fischer

Abstract

Selective demonstration of RNA in tissues was achieved by treating tissue sections with potassium permanganate followed by bisulfite and toluidine blue at pH 1.0 (PBT reaction). It is suggested that this reaction is due to aldehyde groups which are formed by the oxidative cleavage of the pyrimidine rings of RNA which can be selectively demonstrated using bisulfite-toluidine blue as the aldehyde reagent. The specificity of the reaction was tested after RNAase treatment, after acid hydrolysis, and on pure RNA droplets. The aldehyde nature of the reacting groups was checked, after permanganate oxidation, by Schiff's leucofuchsin reagent, and by aldehyde blocking reactions. Two types of intracellular molecular arrangement of RNA molecules could be distinguished by polarization optics after application of the PBT reaction: 1) The strong birefringence, dichroism and metachromatic staining of membrane-bound RNA in ergastoplasm of pancreas, liver and plasma cells indicate a linear (planar) molecular order of RNA molecules on the surface of the membranes, and 2) the isotropic, basophilic staining of RNA not organized in membrane structures (Nissl substance, nucleoli) suggest a random distribution of their dye binding sites.

References

Jan 1, 1977·Acta histochemica·J Fischer, G Romhányi
Sep 1, 1976·Analytical Biochemistry·J D ShaposhnikovY F Bobrov
Nov 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G I Malinin
Jul 15, 1974·Experientia·J C Stockert, O D Colman
May 1, 1969·Journal of Ultrastructure Research·W Bernhard

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