Studies on the organization of the alpha-satellite DNA from African green monkey cells using restriction nucleases and molecular cloning

H GrafH G Zachau


alpha-Satellite DNA from African green monkey cells was analysed with restriction nucleases in some detail confirming and complementing our earlier results. With EcoRI and HaeIII (or BsuRI isoschizomer), about 25 and 10%, respectively, of the satellite DNA were cleaved into a series of fragments of the 172 bp repeat length and multiples thereof. To allow studies with fragments of homogeneous sequence unit length, HindIII fragments were covalently joined with the plasmid pBR 313. After transformation 19 clones were obtained, containing up to three monomer fragments. Nine of the clones were characterized by digestion with EcoRI. Three of these had cleavage sites for this nuclease in the satellite DNA portion. In the six clones tested with HaeIII no cleavage site was detected in the cloned DNA. The results are discussed in relation to the nucleotide sequence data recently published by Rosenberg et al. (1978) and in the context of random and nonrandom processes in satellite DNA evolution


Jan 5, 1975·Journal of Molecular Biology·R J RobertsP A Myers
Apr 28, 1978·Science·H RosenbergM Rosenberg
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B PoliskyH W Boyer
Mar 1, 1977·European Journal of Biochemistry·W Hörz, H G Zachau
Mar 1, 1977·European Journal of Biochemistry·W AltenburgerH G Zachau
Sep 1, 1975·European Journal of Biochemistry·P PhilippsenH G Zachau
May 21, 1976·Analytical Biochemistry·J C Johnson, J T August
May 26, 1975·Analytical Biochemistry·R W ThuringP Borst
May 5, 1975·Journal of Molecular Biology·E M Southern
Dec 4, 1975·Nature·W PfeifferH G Zachau

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