PMID: 11905205Jun 1, 1968Paper

Studies on the permeability of calf thymus nuclei isolated in sucrose

The Journal of Cell Biology
R M Kodama, H TEDESCHI

Abstract

A study of the permeability of calf thymus nuclei isolated in sucrose was carried out with sucrose-14C, glycerol-14C, and carboxydextran-14C (molecular weight, 60,000-90,000). The results indicate that the nuclei are very permeable to both sucrose and glycerol but they exclude the carboxydextran. Results obtained with other low molecular weight non-electrolytes (malonamide-14C, erythritol-14C, D-arabinose-14C, and D-mannitol-14C) are in agreement with the view that the nuclei are freely permeable to these molecular species. A sucrose-impermeable space is also present in these preparations and it has been attributed to the presence of intact cells. The high permeability of nuclei to sucrose was confirmed with Ficoll-separated preparations. The possibility of the presence of a substantial particulate space that allows the penetration of dextran cannot be excluded by these experiments, and this space may correspond to damaged nuclei.

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Citations

Jul 1, 1971·The Journal of Cell Biology·C de Duve
Mar 1, 1973·The Journal of Cell Biology·R NilssonG Dallner
Sep 17, 1968·Biochimica Et Biophysica Acta·R M Kodama, H Tedeschi
Jan 1, 1971·Zeitschrift für allgemeine Mikrobiologie·R May

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