PMID: 9143Sep 7, 1976

Studies on the phosphorylated intermediates of a K+-stimulated ATPase from rabbit gastric mucosa

Biochimica Et Biophysica Acta
T K Ray, J G Forte

Abstract

A density gradient-purified microsomal membrane preparation from rabbit fundic gastric mucosa was used for a detailed study of the K+-stimulated ATPase and associated intermediate reactions. Membranes incubated with gamma-[32P]ATP show the rapid incorporation of 32P into phosphoprotein. Phosphoprotein levels were markedly reduced (1) when ATP hydrolysis went to completion or (2) upon addition of unlabeled ATP, thus suggesting the participation of a rapid turnover phosphorylated intermediate in the gastric microsomal ATPase. Addition of K+, Rb+ or Tl+ greatly reduced the level of the intermediate while stimulating ATPase activity; the observed affinities of these cations were similar for the effects on both ATPase and intermediate levels, with Tl+ greater than K+ greater than Rb+. Neither ATPase nor intermediate were stimulated by Na+, and ouabain was without effect on the reactions, thus differentiating this system from the (Na+ + K+)-ATPase. Addition of various inhibitors showed differential effects on the partial reactions of the gastric ATPase system. N-ethylmaleimide and Zn2+ showed characteristics of completely abolishing the K+-stimulated component of ATPase as well as the effects of K+ in reducing the level of intermedia...Continue Reading

References

Dec 30, 1975·Annals of the New York Academy of Sciences·G SachsH M Sarau
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A M LescureJ F Briat
Sep 18, 1973·Biochemical and Biophysical Research Communications·A L Ganser, J G Forte
Jan 1, 1970·Annual Review of Physiology·R Whittam, K P Wheeler
May 1, 1970·Archives of Biochemistry and Biophysics·G InesiB H McFarland
Nov 1, 1971·Archives of Biochemistry and Biophysics·A S Tanisawa, J G Forte
Dec 23, 1974·Biochemical and Biophysical Research Communications·T K Ray, J G Forte
Jan 1, 1967·Annual Review of Biochemistry·R W Albers
Apr 22, 2010·Journal of the American Chemical Society·Isaac T S Li, Gilbert C Walker

Citations

Aug 1, 1979·The Journal of Clinical Investigation·G SaccomaniG Sachs
Jan 1, 1986·Histochemistry·K FujimotoK Ogawa
Jan 1, 1980·Annals of the New York Academy of Sciences·S L BontingJ J Schrijen
Aug 1, 1988·The Anatomical Record·T SasakiP R Garant
Jan 1, 1981·The Journal of Membrane Biology·E Rabon, G Sachs
Nov 15, 1978·European Journal of Biochemistry·M Lambert, J Christophe
Jan 1, 1989·Drug Metabolism and Drug Interactions·G Saccomani, S J Hersey

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