PMID: 40616Nov 9, 1979

Studies on the possible identity of particulate beta-glucosidase and beta-xylosidase of mouse liver

Biochimica Et Biophysica Acta
M C StephensJ N Kanfer

Abstract

Mouse liver beta-glucosidase (beta-D-glucosidase glucohydrolase, EC 3.2.1.21) and beta-xylosidase (1,4-beta-D-xylan xylohydrolase, EC 3.2.1.37) activities were studied under different conditions of incubation in an attempt to determine whether these two activities are due to a single enzyme or two separate enzymes. The results showed that: (a) Particle-bound beta-glucosidase and beta-xylosidase activities exhibit similar characteristics with different buffers and at various pH values, in the presence or absence of taurocholate. (b) Both activities are inhibited by gluconolactone and conduritol B eposice. beta-Glucosidase activity is inhibited competitively by the two inhibitors, but beta-xylosidase activity is inhibited non-competitively. (c) Xylonolactone was a very poor inhibitor of both activities, but the inhibition of beta-xylosidase activity was more pronounced than that of beta-glucosidase. (d) The presence of glucosides or xylosides simultaneously in the incubation medium suggested the presence of one enzyme with both activities. These results, together with the mode of inhibition produced by gluconolactone and conduritol B epoxide also suggest the presence of two different binding sites for the beta-D-glucoside and bet...Continue Reading

Citations

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y B ChiaoR H Glew
Apr 15, 1978·Archives of Biochemistry and Biophysics·D E Walker, B Axelrod
Jan 1, 1979·Metabolism: Clinical and Experimental·Y B ChiaoR H Glew
Aug 1, 1976·Archives of Biochemistry and Biophysics·S P PetersR H Glew
Jul 1, 1966·The Journal of Clinical Investigation·R O BradyD Shapiro
Oct 1, 1968·Biochemistry·B L ValleeM Sokolovsky
Dec 1, 1964·Analytical Biochemistry·R F ITZHAKI, D M GILL

Related Concepts

Buffers
Particle-bound
xylosides
Beta-Glucosidase
Enzymes, antithrombotic
Xylan 1,4-beta-xylosidase Activity
Xylosidases
Glucosides
Lactones
Conduritol B epoxide

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