Studies on the receptors mediating responses of osteoblasts to thrombin

The International Journal of Biochemistry & Cell Biology
S J SongEleanor J Mackie

Abstract

The serine protease thrombin stimulates proliferation in osteoblasts, but decreases alkaline phosphatase (ALP) activity, a marker of osteoblast differentiation. Three thrombin receptors have been identified, protease activated receptor (PAR)-1, PAR-3 and PAR-4; we have previously demonstrated that mouse osteoblasts express PAR-1 and PAR-4. The effect of thrombin on osteoblast proliferation and differentiation was studied to determine which of the thrombin receptors is responsible for the primary effects of thrombin. Primary mouse calvarial osteoblasts from PAR-1-null and wild-type mice, and synthetic peptides that specifically activate PAR-1 (TFFLR-NH2) and PAR-4 (AYPGKF-NH2) were used. Both the PAR-1-activating peptide and thrombin stimulated incorporation of 5-bromo-2'-deoxyuridine (two to four-fold, P < 0.001) and reduced alkaline phosphatase activity (approximately three-fold, P < 0.05) in cells from wild-type mice. The PAR-4-activating peptide, however, had no effect on either alkaline phosphatase activity or proliferation in these cells. Neither thrombin nor PAR-4-activating peptide was able to affect osteoblast proliferation or alkaline phosphatase activity in cells isolated from PAR-1-null mice. The results demonstrate ...Continue Reading

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Citations

Mar 4, 2005·The American Journal of Pathology·Shu Jun SongEleanor J Mackie
Jan 24, 2009·The Journal of Craniofacial Surgery·Kemal FindikciogluKenan Atabay
Aug 10, 2006·Biological Chemistry·Charles N PagelEleanor J Mackie
Feb 20, 2013·Journal of Molecular Endocrinology·S SivagurunathanE J Mackie
May 21, 2008·The International Journal of Biochemistry & Cell Biology·Kitipong UaesoontrachoonCharles N Pagel
Sep 17, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kaitlin ShenAaron Schindeler
May 22, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandra JastrzebskiJoseph A Lorenzo
Aug 2, 2017·Journal of Dental Research·M M P AlvarezF D Nascimento
Jul 10, 2019·Stem Cells International·Emanuel Silva RovaiMarinella Holzhausen
Dec 29, 2020·Seminars in Thrombosis and Hemostasis·Hanshi Wang, Xizhuang Bai

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