PMID: 3768410Oct 29, 1986Paper

Studies on the role of transferrin and endocytosis in the uptake of Fe3+ from Fe-nitrilotriacetate by mouse duodenum

Biochimica Et Biophysica Acta
R J SimpsonT J Peters

Abstract

Addition of iron-binding proteins (human serum transferrin, mouse serum transferrin, human lactoferrin) to the luminal fluid in tied-off segments of mouse intestine in vivo led to reduced 59Fe3+ absorption from 59Fe3+-nitrilotriacetate when compared to 59Fe3+-nitrilotriacetate alone. Assay of transferrin in luminal fluid from tied segments revealed only trace amounts of immunoreactivity. The levels of luminal transferrin are unaltered in chronic hypoxia where iron absorption is significantly enhanced. Studies in vitro revealed that NH4Cl, dansylcadavarine, para-chloromercuribenzoate and trinitrobenzenesulphonate have no effect on initial 59Fe3+ uptake rates from 59Fe3+-nitrilotriacetate, while N-ethylmaleimide (1 mM) caused a 40% inhibition. In vivo 59Fe3+ uptake was unaffected by preincubation of tied-off segments with colchicine (5 mM) for up to 2 h. These results suggest that receptor-mediated endocytosis of transferrin is not a significant mechanism in the uptake of luminal Fe3+ by mouse duodenum.

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Citations

Nov 13, 2004·The Journal of Nutritional Biochemistry·J M PérèsD Bouglé
Aug 1, 1991·British Journal of Haematology·R J SimpsonT J Peters
Jun 1, 1991·The Journal of Pharmacy and Pharmacology·R J SimpsonP Cremonesi
Aug 15, 2000·Annual Review of Nutrition·M Wessling-Resnick
Jan 1, 1989·The Journal of Clinical Investigation·P WhittakerJ D Cook
Jan 1, 1988·Annals of the New York Academy of Sciences·T J PetersS Snape
Jan 1, 1987·British Journal of Haematology·K R OsterlohT J Peters
Jan 1, 1990·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·A C ProstG Rosselin
Jan 1, 1992·Critical Reviews in Food Science and Nutrition·C E Carpenter, A W Mahoney
Jul 28, 2017·Proceedings. Biological Sciences·Siobhán O'BrienAngus Buckling
Oct 9, 2019·Proceedings. Biological Sciences·Siobhán O'BrienMichael A Brockhurst

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