Studies on the secretion of amino acids and of urea into the gastrointestinal tract of pigs. 3. Secretion of urea determined by continuous intravenous infusion of 15N-urea

Archiv für Tierernährung
H BergnerR Münchmeyer

Abstract

Three pigs, of 34 kg live weight, were each fitted with re-entrant cannulas both in the duodenum and terminal ileum and catheters in the jugular vein and in the carotid artery. Pigs received a diet based on wheat and dried skimmed milk in equal amounts at 12 h intervals. During the preliminary period the digesta flowing from both duodenal and ileal cannulas were collected over 12 h after feeding on two consecutive days and half of them were reintroduced into the gut and half were stored at -20 degrees C. During the experimental period 15N-urea was infused into the jugular vein for 12 hours starting with the morning meal. Total amount of urea infused was 5 g containing 1.22 g 15N-excess. The digesta from both proximal duodenal and ileal cannulas were collected and stored, while the digesta from the preliminary period were reintroduced into the respective distal cannulas. Blood samples were taken at different time of infusion. At the end of infusion period the animals were sacrificed and samples of the contents of the digestive tract and tissues were taken. Urea flux calculated according to atom-% 15N-excess of urea N in plasma was 1.23 to 2.37 g/kg body weight/day. In the duodenal digesta 94.5 +/- 0.2 and in ileal digesta 57.1 +...Continue Reading

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Citations

Jul 3, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·F StumpffJ R Aschenbach
Aug 26, 1998·Annual Review of Nutrition·M F Fuller, P J Reeds
Apr 1, 1989·Archiv für Tierernährung·H Bergner
Oct 28, 2004·American Journal of Physiology. Endocrinology and Metabolism·Cécile BosDaniel Tomé
Jan 1, 1994·Archiv für Tierernährung·H Bergner

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