PMID: 109096May 1, 1979

Studies on the specificity and clinical correlation of antiribosomal antibodies in systemic lupus erythematosus sera

Arthritis and Rheumatism
D KofflerR G Lahita

Abstract

The specificity of antibodies reactive with cytoplasmic ribosomes detected in systemic lupus erythematosus (SLE) sera was studied by a radioimmunoassay procedure with 3H labeled HeLa cell polysomes. The antibodies were directed primarily against RNA or a ribosomal RNA-protein complex (rRNP). Anti-RNA antibodies exhibited comparable reactivity with ribosomal RNA, nuclear RNA, structural RNA, and a slightly lower degree of reactivity with low molecular weight RNA. Anti-rRNP antibodies may be detected by precipitation in agar gel and appear to require both the RNA and protein moieties of ribosomes for reactivity. SLE sera containing only anti-rRNP antibodies are limited to patients with active disease. Sera from patients with both active and inactive SLE contain either anti-RNA or a mixture of anti-RNA and anti-rRNP antibodies. The possible participation of anti-ribosomal antibodies in immune complex formation is discussed.

References

Jul 13, 1973·Biochimica Et Biophysica Acta·I FaifermanM E Kaighn
Jul 1, 1971·The Journal of Experimental Medicine·D KofflerH G Kunkel
Apr 1, 1965·Arthritis and Rheumatism·B C STURGILL, R R CARPENTER
May 13, 1977·Science

Citations

Apr 25, 2007·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·P Staubach-RenzK Steinbrink
Feb 1, 1990·Journal of Steroid Biochemistry·A H Schuurs, H A Verheul
Aug 1, 1985·The Journal of Experimental Medicine·K B ElkonC L Foster
Jun 1, 1992·Molecular Biology Reports·R M Hoet, W J van Venrooij
Jan 1, 1984·Journal of Clinical Immunology·P L MeroniC Zanussi
Dec 14, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kyeong-Hee KimWon-Tae Chung
Mar 31, 1999·The West Indian Medical Journal·N A Hanchard
Jan 30, 2007·Seminars in Arthritis and Rheumatism·Gisele Zandman-GoddardYehuda Shoenfeld
Dec 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K B ElkonN Brot
Jan 24, 2006·Clinical and Vaccine Immunology : CVI·M MahlerM J Fritzler
Jul 1, 1982·Arthritis and Rheumatism·I Faiferman, D Koffler
Aug 1, 1986·Arthritis and Rheumatism·E Bonfa, K B Elkon

Related Concepts

Antibody Specificity
Fluorescent Antinuclear Antibodies
Autoantibodies
Immunodiffusion Measurement
HeLa Cells
Liver
Lupus Erythematosus, Systemic
Radioimmunoassay
Ribonucleoproteins
Ribosomes

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