PMID: 1195340Apr 23, 1975Paper

Studies on the structure of milk fat globule membrane

The Journal of Membrane Biology
I H Mather, T W Keenan

Abstract

Milk fat globule membrane was solubilized with sodium dodecyl sulfate and mercaptoethanol and the membrane proteins were separated by SDS-polyacrylamide gel electrophoresis. The membrane preparations contained three major size classes of polypeptide of 155,000, 62,500 and 43,500 daltons. At least five glycopeptides were separated of which two stained intensely with periodic acid-Schiff reagent, but poorly with coomassie blue. Trypsin hydrolysis of whole cream and isolated milk fat globule membrane revealed major differences in the rates of protein hydrolysis. Many of the membrane proteins of whole cream resisted proteolysis compared with the same proteins in the isolated membrane. Two glycopeptides were resistant to trypsin digestion in either preparation. Treatment of whole cream with neuraminidase led to the release of at least 70% of the protein-bound sialic acid. Whole cream and isolated membrane samples were iodinated with 125I in the presence of lactoperoxidase and hydrogen peroxide. The membrane proteins were significantly more accessible to lactoperoxidase-125I i in isolated membrane compared with the proteins of whole cream. Polypeptides of molecular weight 43,500 and approximately 48,000 daltons were predominantly lab...Continue Reading

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Citations

Jan 1, 1979·The International Journal of Biochemistry·C J HofmannW N Eigel
Jan 1, 1989·The International Journal of Biochemistry·H M Valivullah, T W Keenan
Apr 1, 1993·The International Journal of Biochemistry·B H KeonT W Keenan
Feb 1, 1996·Comparative Biochemistry and Physiology. Part A, Physiology·C A Gibson, C R Baumrucker
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L R MurrayT W Keenan
Jun 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E W Bingham, E L Malin
Sep 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W M CampanaS Patton
Aug 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M L HuottS Patton
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Apr 23, 2003·Advanced Drug Delivery Reviews·James L McManaman, Margaret C Neville
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Sep 5, 2002·Free Radical Biology & Medicine·Roger Harrison
Aug 1, 1992·The Journal of Dairy Research·A V HoulihanC J Masters
Aug 13, 2013·Journal of Agricultural and Food Chemistry·Haotian ZhengDavid W Everett
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Sep 1, 1985·Journal of Dairy Science·C R Baumrucker

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