PMID: 2510601Nov 15, 1989Paper

Studies on the structure of NADH: ubiquinone oxidoreductase complex: topography of the subunits of the iron-sulfur protein component

Archives of Biochemistry and Biophysics
A L HanY Hatefi

Abstract

Resolution of the mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) by chaotropic agents result in the separation of three building blocks of the enzyme, designated FP (flavoprotein), IP (iron-sulfur protein), and HP (hydrophobic protein). FP contains three subunits of Mr 51, 24, and 9 kDa; one FMN; and two iron-sulfur clusters. Immunochemical studies with monospecific antibodies to the FP subunits have indicated that all three subunits of FP protrude from the inner mitochondrial membrane on the matrix side, whereas no reactive epitopes from these subunits were found exposed on the cytosolic side [A.-L. Han, T. Yagi, and Y. Hatefi (1988) Arch. Biochem. Biophys. 267, 490-496]. IP contains six subunits of Mr 75, 49, 30, 18, 15, and 13 kDa and four iron-sulfur clusters. In the present study, immunochemical experiments (enzyme-linked immunosorbent assays and 125I-protein A labeling) were carried out with monospecific antibodies to the above IP subunits and with bovine Complex I, submitochondrial particles, mitoplasts, and intact mitochondria as sources of antigens. Results have indicated that all six IP subunits protrude from the inner mitochondrial membrane into the matrix, and that the 75-kDa subunit, and possibly ...Continue Reading

References

Feb 1, 1979·Archives of Biochemistry and Biophysics·Y M Galante, Y Hatefi
Apr 1, 1988·Trends in Biochemical Sciences·R A Capaldi
Jan 1, 1985·Annual Review of Biochemistry·Y Hatefi
Feb 1, 1987·The Journal of Clinical Investigation·R W MoreadithA L Lehninger
Mar 1, 1988·Annals of Neurology·T IchikiY Wada
Dec 1, 1988·The Biochemical Journal·S D Patel, C I Ragan
Jan 1, 1986·Annals of the New York Academy of Sciences·E Carafoli
Jun 1, 1985·Annals of Neurology·S DiMauroD C DeVivo
Jul 15, 1983·European Journal of Biochemistry·H von Bahr-LindströmH Jörnvall

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Citations

Dec 1, 1994·Journal of Molecular Recognition : JMR·J OvádiH O Spivey
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·M Finel
May 8, 1991·European Journal of Biochemistry·H WeissD Preis
Feb 8, 1993·Biochimica Et Biophysica Acta·T Yagi
May 3, 2005·Biochimica Et Biophysica Acta·Isabel MarquesArnaldo Videira
Sep 15, 2007·Biochimica Et Biophysica Acta·Rutger O VogelLeo G J Nijtmans
Sep 2, 2021·Journal of Chemical Theory and Computation·Werner DobrautzGiovanni Li Manni
Dec 6, 2001·Journal of the American Chemical Society·T LovellL Noodleman

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