Studies on the structure of the organ-specific determinant of human colonic mucin.

Molecular Immunology
D V Gold, D Shochat

Abstract

We had previously shown that the human colon produces at least two immunochemically distinct mucins, one neutral and the other a sialomucin [Gold et al. J. biol. Chem. 256, 6354-6358 (1981)]. In addition, the sialomucin was shown to contain an immunodeterminant restricted to colonic epithelium and may thus prove useful as a tissue-specific marker. In the current study we have shown that a specific linkage of sialic acid to the oligosaccharide backbone has a major role in the organ-specific immunodeterminant structure. Treatment of intact colonic mucin with sialidase (Cl. perfringens) cleaved 20-80% of the sialic acid as measured colorimetrically. Immunoreactivity was decreased by 0-42% with respect to the untreated material. Saponification (0.1 N KOH, 20 min at room temp) caused an approximate 90% decrease in immunoreactivity for each mucin. Subsequent to saponification, neuraminidase cleaved most of the sialic acid from the mucins. The majority of sialic acid was observed to be O-acetylated, thus making it sialidase-insensitive. Gas chromatography-mass spectrometric analyses of the trimethylsilyl sialic acid derivatives indicated the presence of NeuNAc; NeuNAc, 9-OAc; and NeuNAc, 7,9 diOAc as the major sialyl derivatives. The ...Continue Reading

References

Apr 1, 1978·Tissue Antigens·D V Gold, F Miller
Jul 1, 1978·Carbohydrate Research·H E CarlssonJ Lönngren
Jan 27, 1955·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H FLOREY

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Citations

Dec 1, 1994·Critical Reviews in Oncology/hematology·T LesuffleurF X Real
Feb 13, 2002·Hybridoma and Hybridomics·D V Gold, T M Cardillo
Mar 1, 1995·European Journal of Biochemistry·J MauryS Maroux
Aug 31, 1990·Biochemical and Biophysical Research Communications·J R GumY S Kim
Apr 15, 1994·International Journal of Cancer. Journal International Du Cancer·D V GoldT Cardillo

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