Studies on the structure, regulation, and pathogenic potential of anti-dsDNA antibodies

Methods : a Companion to Methods in Enzymology
L SpatzB Diamond

Abstract

Studies of anti-double-stranded (anti-ds)DNA antibodies have provided insights into how and why these antibodies arise in systemic lupus erythematosus. In this review we discuss the experimental approaches that have been used by our laboratory to study these autoantibodies. Structure/function analyses including site-directed mutagenesis have helped characterize the molecular genetics of anti-dsDNA antibodies, and more recently peptide libraries have been used to define molecular motifs that these antibodies bind. Most of the pathogenic anti-dsDNA antibodies observed in lupus are somatically mutated. We demonstrated in vitro and in vivo that anti-bacterial antibodies can mutate to acquire specificity for dsDNA. Furthermore, using a fusion partner constitutively expressing bcl-2, NSO(bcl-2), we have shown the existence of anergic or preapoptotic B cells making antibodies that cross-react with both bacterial antigen and dsDNA. Whether defects in the regulation of these antibodies might contribute to serum expression of anti-dsDNA antibodies in some individuals remains unknown. A major emphasis of this review is the regulation of anti-dsDNA antibodies in a transgenic mouse model harboring the gene for the heavy chain of a pathogeni...Continue Reading

Citations

Feb 24, 2010·Chemical Communications : Chem Comm·Francesco RicciGiuseppe Palleschi
May 14, 2011·The Journal of Biological Chemistry·Sanjeev KumarDavid A Isenberg
Feb 6, 2004·The Journal of Experimental Medicine·Zhiyan LiangChandra Mohan
Jul 8, 2008·Veterinary Immunology and Immunopathology·Nandakumar PuliyathRose G Mage
Sep 26, 2007·Annals of the New York Academy of Sciences·Mirjana PavlovicJames X Hartmann
Apr 15, 2005·Molecular Immunology·Christine M GrimaldiBetty Diamond
Dec 15, 2010·Autoimmune Diseases·Mirjana PavlovicYehuda Shoenfeld
Jun 26, 2007·The Journal of Biological Chemistry·Kazuyo ToyodaKoji Uchida
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K D Klonowski, M Monestier
May 6, 1999·European Journal of Immunology·M S BynoeB Diamond
Nov 7, 2017·BioNanoScience·Tatiana A NevzorovaRustem I Litvinov
Jan 22, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·John G RoutsiasHaralampos M Moutsopoulos

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.