Studies on the sugar chains of interferon-gamma from human peripheral-blood lymphocytes

Journal of Biochemistry
S YamamotoT Ikenaka

Abstract

Sugar chains of interferon-gamma (IFN-gamma) from human peripheral-blood lymphocytes (PBL) were liberated by hydrazinolysis. After N-acetylation, the reducing end residues of the sugar chains were tagged with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and reversed-phase HPLC. Five major PA-sugar chains were obtained. The structure of each PA-sugar chain was estimated by comparing its elution times on anion exchange, reversed-phase, and size-fractionation HPLC with those of PA-sugar chains of IFN-gamma from the human myelomonocyte cell line HBL-38 (Yamamoto, S. et al. (1989) J. Biochem. 105, 547-555) as standards, and also by comparison of their elution times after partial desialylation. The results showed that IFN-gamma (PBL) contained mono- and disialo-biantennary structures with 0 or 1 mol of fucose residue, as found for IFN-gamma (HBL-38), but the N-acetylneuraminyl alpha 2-6 linkage was dominant in IFN-gamma (PBL), unlike IFN-gamma (HBL-38), which contains both N-acetylneuraminyl alpha 2-3 and alpha 2-6 linkages.

Citations

Apr 15, 2000·Glycobiology·K FukutaT Makino
Jun 1, 1992·Journal of Interferon Research·K CantellI Julkunen
Dec 15, 1993·European Journal of Biochemistry·A YonedaN Seno
Jul 9, 2002·Clinical and Experimental Immunology·M HollandR Jefferis
Jun 15, 1995·European Journal of Biochemistry·H OgawaI Matsumoto
Apr 27, 2019·Journal of Molecular Modeling·Elena LilkovaLeandar Litov
Aug 21, 2003·Biochimica Et Biophysica Acta·Shinji TakamatsuTadashi Makino

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