PMID: 737191Dec 22, 1978Paper

Studies on the thromboxane synthesizing system in human platelet microsomes

Biochimica Et Biophysica Acta
H H Tai, B Yuan

Abstract

The thromboxane synthesizing system from human platelets has been characterized by using arachidonic acid and prostaglandin endoperoxide as indirect and direct substrates. The synthesis of thromboxanes from either substrates catalyzed by the microsomal fraction was monitored by measuring the formation of thromboxane B2 immunoreactivity. Both hemoglobin and phenolic compounds were required for the maximal synthesis of thromboxane B2 from arachidonic acid but not from prostaglandin endoperoxide. Studies on the kinetics of the formation of thromboxane B2 from either substrates indicated that the synthesis of prostaglandin endoperoxide intermediate is the rate-limiting step in the overall production of thromboxanes from arachidonic acid. Effect of the microsomal protein concentrations on the rate of formation of thromboxane B2 showed concave upward relationship suggesting the possible involvement of endogenous stimulator(s) in thromboxane synthetase catalyzed reaction. A variety of compounds including sulfhydryl inhibitors, prostaglandin endoperoxide analog, prostaglandin antagonist, N-0164, and N-substituted imidazoles could directly inhibit the thromboxane synthetase, while nonsteroidal antiinflammatory drugs apparently affected ...Continue Reading

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P NeedlemanM Minkes
Apr 1, 1977·Prostaglandins·S MoncadaP Needleman
Jul 1, 1978·Analytical Biochemistry·H H Tai, B Yuan
Jan 13, 1978·Biochemical and Biophysical Research Communications·H H Tai, B Yuan
Feb 21, 1977·Biochemical and Biophysical Research Communications·F F Sun
Jun 7, 1976·Biochemical and Biophysical Research Communications·L S WolfeJ Marion
Jul 1, 1976·Analytical Biochemistry·H H Tai, W Y Chey
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Dec 11, 1974·Biochemical and Biophysical Research Communications·M Hamberg, B Samuelsson
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Dec 20, 1973·Biochimica Et Biophysica Acta·D H Nugteren, E Hazelhof

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Citations

Sep 1, 1982·Lipids·H KawaguchiY Imai
Apr 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·B Damtew, P J Spagnuolo
Jun 1, 1980·Prostaglandins and Medicine·C L Tai, H H Tai
Jul 31, 1985·Biochemical and Biophysical Research Communications·A Mobley, H H Tai

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