PMID: 6965930Jan 1, 1980Paper

Studies on the valyl-h-tRNA synthetase of chick embryo brain irradiated with 60Co gamma-rays, in vivo

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
E Bölöni

Abstract

18-day-old chick embryos (Leghorn) were irradiated in vivo with 1,2,3,4,5 or 7 Gy 60Co gamma-rays (dose rate = 0.9 Gy/min). Twenty-four hours after irradiation the activity of valyl-tRNA synthetase isolated from the brains was determined and compared with that of the non-irradiated control. The aminoacylation activity was found to decrease exponentially as a function of the dose (D37 = 6 Gy). Irradiation caused a more pronounced decrease in valyl-tRNA synthetase activity in 18-day-old than in 14-day-old chick embryos and induced in the valine-dependent ATP-PPi exchange reaction less change than in the valyl-tRNA FORMATion. The exposure of 15-day-old chick embryos to a dose of 4 or 5 Gy induced, respectively, a 40 or 20 per cent increase in enzyme activity of VRS (prepared on day 19 of embryonic life) relative to the control. In these experiments a decrease of Km value for tRNA has been found. The change in the number of sulfhydryl groups was also investigated.

References

Sep 15, 1977·The Biochemical Journal·D McEwen NichollsW Sendecki
Nov 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·J P KushnerS Dickman
Feb 1, 1970·The Biochemical Journal·E J HidvégiV Várterész
Oct 1, 1968·The Biochemical Journal·E J HidvégiV Várterész
Jan 15, 1972·FEBS Letters·U Del Monte, G Cini
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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