PMID: 6170335Sep 15, 1981Paper

Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas

Biochimica Et Biophysica Acta
T Miyagi, S Tsuiki

Abstract

When homogenates of rat liver and hepatomas were centrifuged at 78 000 X g, over 90% of liver N-acetylglucosaminyltransferase assayed with beta-galactosidase- and beta-N-acetylhexosaminidase-treated asialofetuin as acceptor was recovered in the particulate fraction, while as much as 24% of hepatoma transferase was in the supernatant fraction. The particulate transferase solubilized by 0.2% sodium deoxycholate emerged from a DEAE-cellulose column at 0.04 M NaCl (transferase A). The supernatant fractions from all the hepatomas tested contained a second N-acetylglucosaminyltransferase eluted from the column at 0.02 M NaCl (transferase B). Transferase B was absent from liver supernatant fraction. The activities of these transferases toward various acceptors and the effect of beta-N-acetylhexosaminidase on their products suggest that both transferases are UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase. Although ovalbumin and glycopeptide V, which was isolated from pronase digest of ovalbumin, were good acceptors, transferase A utilized ovalbumin and glycopeptide V with apparent Km values of 0.44 and 0.33 mM, respectively, whereas the corresponding values for transferase B were 4.5 and 0.050 mM.

References

Sep 9, 1977·Biochemical and Biophysical Research Communications·T TaiA Kobata
May 1, 1979·The Tohoku Journal of Experimental Medicine·M Koseki, K Tsurumi
Aug 3, 1978·Biochimica Et Biophysica Acta·Y OkamotoN Akamatsu
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·S KORNFELDP J O'BRIEN

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Citations

Sep 1, 1988·Archives of Biochemistry and Biophysics·S TamuraS Tsuiki
Feb 1, 1990·Japanese Journal of Cancer Research : Gann·R ShinehaS Tsuiki
Jan 1, 1988·Japanese Journal of Cancer Research : Gann·J SagawaS Tsuiki
Sep 1, 1990·Japanese Journal of Cancer Research : Gann·T MiyagiS Tsuiki
Jan 1, 1989·Critical Reviews in Oncology/hematology·J A Alhadeff

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