Study by optical spectroscopy and molecular dynamics of the interaction of acridine-spermine conjugate with DNA

Biophysical Chemistry
S Sánchez-CarrascoF Javier Ramírez

Abstract

We report a spectroscopic and theoretical study of the interaction between double-stranded oligonucleotides containing either adenine-thymine or guanine-cytosine alternating sequences and N1-(Acridin-9-ylcarbonyl)-1,5,9,14,18-pentazaoctadecane, or ASC, which is formed by the covalent bonding of spermine and 9-amidoacridine moieties via a trimethylene chain. Solutions containing the oligonucleotides and the conjugate at different molar ratios were studied using complementary spectroscopic techniques, including electronic absorption, fluorescence emission, circular dichroism, and Raman spectroscopy. The spectroscopical properties of ASC at both the vibrational and the electronic levels were described by means of ab initio quantum-chemical calculations on 9-amidoacridine, used as a model compound. Molecular dynamics calculations, based on the QM/MM methodology, were also performed using previously docked structures of two oligonucleotide-ASC complexes containing the A-T and the G-C sequence. Our data, taken all together, allowed us to demonstrate that conjugation of spermine to acridine modulates and gives additional properties to the interaction of the latter with DNA. As the ASC molecule has a high affinity by the polyamine tran...Continue Reading

References

Nov 1, 1992·Investigational New Drugs·N T SklarinP J Rosen
Oct 9, 1984·Biochemistry·D Porschke
Apr 1, 1995·Protein Engineering·I A Vakser
Feb 4, 1998·Biophysical Journal·E RaspaudF Livolant
Jun 15, 1992·Physical Review. B, Condensed Matter·J P Perdew, Y Wang
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Jul 11, 2001·Biochemical and Biophysical Research Communications·J Ruiz-ChicaF J Ramírez
Sep 20, 2001·Current Pharmaceutical Design·M DemeunynckA Martelli
May 16, 2002·Bioconjugate Chemistry·Andrew J GeallIan S Blagbrough
Nov 1, 2002·Journal of Medicinal Chemistry·Jean-Guy DelcrosPhilippe Uriac
Jul 31, 2003·Pharmacology & Therapeutics·Annette K LarsenAndrzej Skladanowski
Oct 3, 2003·Journal of Medicinal Chemistry·R John HarrisonStephen Neidle
Jan 31, 2004·Nucleic Acids Research·A J Ruiz-ChicaF J Ramírez
Aug 4, 2004·Amino Acids·U Bachrach
Dec 1, 2004·Biochemistry·Nathalie DiasChristian Bailly
Apr 2, 2005·Nature Reviews. Cancer·Stephen Neidle, David E Thurston

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Citations

Mar 14, 2013·ACS Chemical Biology·Amin Haghighat JahromiSteven C Zimmerman
Jan 29, 2011·Chemistry : a European Journal·Paula López-SenínVicente Marchán
Aug 6, 2013·The Journal of Physical Chemistry. B·Anik SenBishwajit Ganguly
Sep 22, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F AhmadiM Khodadadian
Apr 13, 2010·The Journal of Physical Chemistry. B·Giovanni M PavanAndrea Danani

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