Study of a corn (Zea mays L.) mutant (blue fluorescent-1) which accumulates anthranilic acid and its beta-glucoside.

Biochemical Genetics
M Singh, J M Widholm

Abstract

A corn (Zea mays L.) mutant, blue fluorescent-1 (bf), is described that shows ultraviolet light induced blue fluorescence in young seedling leaves if homozygous for the mutant gene, and in anthers if either homozygous or heterozygous. The blue fluorescent compounds were extracted with acetone and separated by paper chromatography. Anthranilic acid was present and the beta-glucoside was also identified by paper chromatography and beta-glucosdase and acid treatment. A third major fluorescent compound was not identified, but it was convertible to anthranilic acid by acid treatment. Anthranilate synthetase from mutant plants was 3-40 times more active and was also more resistant to feedback inhibition by tryptophan than was the enzyme from normal plants. The high activity and feedback resistance would both lead to anthranilate accumulation. Anthranilate-phosphoribosylpyrophosphate phosphoribosyltransferase (PR transferase), the enzyme which usually utilizes anthranilate in the tryptophan pathway, was inhibited by the beta-glucoside of anthranilic acid in a noncompetitive manner and showed very little activity in the mutant plant extract. This inhibition of the enzyme which utilizes anthranilate would also lead to accumulation. Appa...Continue Reading

References

Oct 8, 1968·Biochimica Et Biophysica Acta·D P Delmer, S Mills
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·I P Crawford, I C Gunsalus
Oct 9, 1964·Biochimica Et Biophysica Acta·J M EDWARDSJ S SHANNON
Oct 1, 1951·Proceedings of the National Academy of Sciences of the United States of America·H J Teas, E G Anderson
Jan 1, 1968·Plant Physiology·D P Delmer, S E Mills
Nov 1, 1968·Plant Physiology·M Kutacek, A W Galston

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Citations

May 1, 1995·Phytochemistry·R M RomeroJ D Phillipson

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