Study of a humanized inhibitory anti-platelet glycoprotein VI phage antibody from a phage antibody library

Hematology
Qinghong LiuMing Hou

Abstract

Objective The aims of the study were to study the effect of anti-platelet glycoprotein (GP) VI auto-antibodies on platelet aggregation and use phage surface display technology to produce anti-platelet GPVI phage antibody fragment, which may be developed to inhibit platelet aggregation in the treatment of cardiovascular disease. Methods Plasma samples from patients with immune thrombocytopenia (ITP) were screened by monoclonal antibody immobilization of the platelet antigen assay and the platelet aggregation test for anti-platelet GPVI auto-antibody with an inhibitory effect. The humanized anti-platelet GPVI phage antibody was produced by phage surface display technology. The function of the phage antibody fragment against platelet aggregation was examined by the platelet aggregation test. Results Of 726 ITP patients, 2 (0.27%) patients' plasma significantly inhibited platelet aggregation induced by collagen-1. After five rounds of selection, enrichment, and purification, a soluble phage antibody fragment was produced, which can inhibit platelet aggregation induced by collagen-1. The results demonstrate that only a few of the screened anti-platelet GPVI auto-antibodies showed an inhibitory effect on platelet aggregation. Discuss...Continue Reading

References

Jul 31, 1995·Journal of Biotechnology·M LittleM Braunagel
Mar 31, 1999·Baillière's Clinical Haematology·H DeckmynK Peerlinck
Mar 22, 2003·Blood·Bernhard Nieswandt, Steve P Watson
Jul 3, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Christelle LecutJohan W M Heemskerk
Sep 9, 2004·Seminars in Thrombosis and Hemostasis·Mark L Kahn
Jun 22, 2006·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Xiaoxia ChuDaoxin Ma
Dec 12, 2007·Vox Sanguinis·C KaplanUNKNOWN International Society of Blood Transfusion--Working Party on Platelet Immunology
Feb 11, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Urartu Ozgur Safak Seker, Hilmi Volkan Demir
Jul 26, 2011·Biotechnology Journal·John Löfblom
Mar 9, 2012·Molecular and Cellular Biochemistry·Bipasha DeyVibha Rani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
Xue-Bin JiLi-zhen Li
Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
Xiao-Xia ChuDao-Xin Ma
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ning-Zheng DongChanggeng Ruan
© 2021 Meta ULC. All rights reserved