PMID: 7032912Nov 1, 1981Paper

Study of a zone highly sensitive to proteases in flavocytochrome b2 from Saccharomyces cerevisiae

European Journal of Biochemistry
R Ghrir, F Lederer

Abstract

Flavocytochrome b2 from baker's yeast is a bifunctional tetrameric protein which carries two prosthetic groups, FMN and heme, per subunit of Mr 58 000. The amino terminus of the subunit is wrapped around the heme and constitutes the so-called cytochrome b2 core (Mr 11 000), homologous to cytochrome b5. It has been shown in the past that a number of proteases (yeast proteases, chymotrypsin) preferentially cleave the peptide chain at a point situated much further down the polypeptide chain than the C terminus of the heme-binding domain. Some enzymatic parameters are concomitantly modified, but not the quaternary structure. This paper describes the conditions for selective proteolysis of intact flavocytochrome b2 and of its various previously studied stable nicked forms by the protease from Staphylococcus aureus V8. Successive attack by a combination of two proteases is also described. We have established the amino acid sequence of the area where proteolytic attack takes places, and shown that chymotrypsin and S. aureus protease open only one bond, whereas yeast proteases remove five residues from the central part. The various nicked forms, some of which have lost up to 16 amino acid residues, have been enzymatically characterized...Continue Reading

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Citations

Jan 1, 1994·Protein Science : a Publication of the Protein Society·A Balme, F Lederer
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Z X XiaF S Mathews
Jan 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jean Marie BourhisYlva Lindqvist
Mar 15, 1983·European Journal of Biochemistry·D Pompon, F Lederer
Apr 5, 1988·European Journal of Biochemistry·P Urban, F Lederer
Dec 15, 1988·European Journal of Biochemistry·G A ReidS K Chapman
May 26, 2016·Physical Chemistry Chemical Physics : PCCP·N GilletV Moliner
Oct 15, 1985·European Journal of Biochemistry·F LedererL Perez
Apr 20, 1990·Journal of Molecular Biology·Z X Xia, F S Mathews

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