Study of an RNA helicase implicates small RNA-noncoding RNA interactions in programmed DNA elimination in Tetrahymena.

Genes & Development
Lucia AronicaKazufumi Mochizuki

Abstract

Tetrahymena eliminates micronuclear-limited sequences from the developing macronucleus during sexual reproduction. Homology between the sequences to be eliminated and approximately 28-nucleotide small RNAs (scnRNAs) associated with an Argonaute family protein Twi1p likely underlies this elimination process. However, the mechanism by which Twi1p-scnRNA complexes identify micronuclear-limited sequences is not well understood. We show that a Twi1p-associated putative RNA helicase Ema1p is required for the interaction between Twi1p and chromatin. This requirement explains the phenotypes of EMA1 KO strains, including loss of selective down-regulation of scnRNAs homologous to macronuclear-destined sequences, loss of H3K9 and K27 methylation in the developing new macronucleus, and failure to eliminate DNA. We further demonstrate that Twi1p interacts with noncoding transcripts derived from parental and developing macronuclei and this interaction is greatly reduced in the absence of Ema1p. We propose that Ema1p functions in DNA elimination by stimulating base-pairing interactions between scnRNAs and noncoding transcripts in both parental and developing new macronuclei.

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Citations

Oct 23, 2009·Genetica·Xavier ThomasSylvaine Renault
Sep 15, 2011·The Journal of Biological Chemistry·Ursula E Schoeberl, Kazufumi Mochizuki
Dec 24, 2008·Nucleic Acids Research·Gersende LepèreEric Meyer
Aug 17, 2012·Genes & Development·Shan Gao, Yifan Liu
Nov 23, 2011·Development, Growth & Differentiation·Kazufumi Mochizuki
Jan 25, 2011·Eukaryotic Cell·Jacek K NowakMireille Bétermier
Jul 15, 2009·Molecular and Cellular Biology·Janna BednenkoMartin A Gorovsky
Jan 10, 2008·Annual Review of Cell and Developmental Biology·Travis Thomson, Haifan Lin
Jul 16, 2010·BMC Microbiology·Clara Jana-Lui BuschKazufumi Mochizuki
Jul 11, 2009·Genome Biology·Douglas L Chalker
Jan 1, 2010·Yi chuan = Hereditas·Zhao-Hui Xie
Feb 24, 2010·Yi chuan = Hereditas·Zhao-Hui Xie
Aug 2, 2011·Annual Review of Genomics and Human Genetics·Mariusz NowackiLaura F Landweber
Sep 14, 2011·Annual Review of Genetics·Douglas L Chalker, Meng-Chao Yao
Jan 27, 2015·International Journal of Molecular Sciences·Ana Lúcia LeitãoFrancisco J Enguita
Jul 19, 2011·Current Opinion in Genetics & Development·Hirotsugu IshizuHaruhiko Siomi
Mar 23, 2011·Current Opinion in Plant Biology·Karl F Erhard, Jay B Hollick
Sep 7, 2013·Science China. Life Sciences·Xuezhu Feng, Shouhong Guang
Nov 3, 2009·Current Opinion in Microbiology·Mariusz Nowacki, Laura F Landweber
Dec 22, 2015·Developmental Cell·Kensuke Kataoka, Kazufumi Mochizuki
Nov 20, 2015·Seminars in Cell & Developmental Biology·Robyn S M Lim, Toshie Kai
Oct 7, 2015·Wiley Interdisciplinary Reviews. RNA·Daniel Gebert, David Rosenkranz
Feb 23, 2012·Biology of the Cell·Robert S CoyneSandra Duharcourt
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Kazufumi Mochizuki
Sep 9, 2010·Essays in Biochemistry·Kazufumi Mochizuki
Jun 1, 2011·Research in Microbiology·Eduardo OriasEileen P Hamilton
Jul 14, 2010·Journal of Microbiological Methods·Kensuke KataokaKazufumi Mochizuki
Jun 23, 2015·Molecular Cell·Tomoko NotoKazufumi Mochizuki

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