Study of Bone Marrow Mesenchymal and Tendon-Derived Stem Cells Transplantation on the Regenerating Effect of Achilles Tendon Ruptures in Rats

Stem Cells International
Mohanad Kh Al-AniLi Yang

Abstract

Comparative therapeutic significance of tendon-derived stem cells (TDSCs) and bone marrow mesenchymal stem cells (BMSCs) transplantation to treat ruptured Achilles tendon was studied. Three groups of SD rats comprising 24 rats each, designated as TDSCs and BMSCs, and nontreated were studied for regenerative effects through morpho-histological evaluations and ultimate failure load. For possible mechanism in tendon repair/regeneration through TDSCs and BMSCs, we measured Collagen-I (Col-I), Col-III gene expression level by RT-PCR, and Tenascin-C expression via immunofluorescent assay. TDSCs showed higher agility in tendon healing with better appearance density and well-organized longitudinal fibrous structure, though BMSCs also showed positive effects. Initially the ultimate failure load was considerably higher in TDSCs than other two study groups during the weeks 1 and 2, but at week 4 it attained an average or healthy tendon strength of 30.2 N. Similar higher tendency in Col-I/III gene expression level during weeks 1, 2, and 4 was observed in TDSCs treated group with an upregulation of 1.5-fold and 1.1-fold than the other two study groups. Immunofluorescent assay revealed higher expression of Tenascin-C in TDSCs at week 1, whil...Continue Reading

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Citations

Oct 6, 2016·Annals of the New York Academy of Sciences·Daniel J Leong, Hui B Sun
May 5, 2017·Journal of Cellular and Molecular Medicine·Stefano TestaCesare Gargioli
Feb 27, 2018·Tissue Engineering. Part a·Mark L WangAndrzej Fertala
Feb 5, 2019·Journal of Cellular Physiology·Yan-Jing ZhangTing-Wu Qin
Jun 30, 2019·International Journal of Molecular Sciences·Raquel Costa-AlmeidaManuela E Gomes
Oct 10, 2020·World Journal of Stem Cells·Hao-Nan WangGuo-Xin Ni
Aug 23, 2017·EFORT Open Reviews·Fan WuDenitsa Docheva

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