Study of CCM Microvascular Endothelial Phenotype by an In Vitro Tubule Differentiation Model.

Methods in Molecular Biology
Simona Delle Monache, Saverio Francesco Retta

Abstract

Cerebral cavernous malformation (CCM) proteins play critical roles for endothelial cell functions, including cytoskeletal remodeling, cell-cell interactions, cell polarity, tube formation, and angiogenesis. It has been shown that the mutation of even one of the CCM genes involved in CCMs can determine an alteration in the angiogenesis process, but the precise mechanism is yet to be clarified.Here using a model of cerebral microvascular endothelial cells (hBMEC) transiently silenced by CCM1, we tried to mimic the physiological conditions that occur in the presence of CCM1 gene know-down evaluating their ability to form tube structures through an in vitro angiogenesis assay.

Related Concepts

Related Feeds

Cavernous Hemangioma

Cavernous hemangioma is a blood vessel defect or benign tumor that leads to leakage of blood to the surrounding tissues. This can occur in several organs including the brain, which can lead to seizures. Discover the latest research on cavernous hemangiomas here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Joycelyn WüstehubeAndreas Fischer
Methods in Molecular Biology
Simona Delle Monache, Saverio Francesco Retta
Current Neurology and Neuroscience Reports
Nicholas W PlummerDouglas A Marchuk
© 2022 Meta ULC. All rights reserved