PMID: 6971904Apr 1, 1981Paper

Study of congenitally immunologic mutant New Zealand mice. V. B cell function of NZB-Xid mice

Journal of Immunogenetics
Y OhsugiA Ahmed

Abstract

NZB mice bearing the CBA/N X chromosome linked defect were generated by repetitive backcrossing and selection of the Xid gene. The male offspring resulting from the cross of NZB with CBA/N were selected as being XidY on the basis of sera IgM and IgG3 levels and responsiveness to DNP-Lys-Ficoll. Following this inbreeding protocol, 6th generation backcross NZB XidY mice were compared to littermate controls with respect to B cell function. Sera immunoglobulin levels of IgG1, IgG2b and IgA were similar in XidY and XY mice. In contrast, levels of IgM and IgG3, from XidY mice were approximately 15% and 50%, respectively, of values found in littermates. Furthermore, XidY mice failed to respond to DNP-Lys-Ficoll and had less than 3% splenic Lyb 5.1-bearing cells. Splenic immunoglobulin cell surface profiles, obtained by the fluorescent activated cell sorter, indicated a significant reduction in the frequency of Ig bearing cells in Xid animals. Such profiles were similar to those obtained for spleen cells from reference control CBA/N mice. Finally, an elevated number of splenic, lymph node and bone marrow background and lipopolysaccharide-induced B cell clones in semi-solid phase agar was found in NZB but not C57BL/6, C3H, BALB/c and DB...Continue Reading

References

Jun 1, 1978·Arthritis and Rheumatism·N L Warner
Jul 1, 1979·The Journal of Experimental Medicine·J D TaurogA D Steinberg
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P B NakajimaB T Huber
Jan 1, 1978·Arthritis and Rheumatism·A S CohenM Skinner
May 1, 1976·The Journal of Experimental Medicine·P W KincadeM A Moore
Sep 30, 1976·Nature·E S RavecheA D Steinberg

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Citations

Jan 4, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·S K LundyN W Lukacs
Mar 1, 1984·Cellular Immunology·D S PisetskyA D Steinberg
Jun 1, 1982·The Journal of Experimental Medicine·H JyonouchiM E Gershwin
Jan 1, 1982·Annals of the New York Academy of Sciences·F DumontA Ahmed
Jan 1, 1982·Immunological Reviews·P W KincadeG Lee
May 4, 1992·Annals of the New York Academy of Sciences·T ShiraiS Hirose

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