Study of cytotoxic and therapeutic effects of stable and purified silver nanoparticles on tumor cells.

Nanoscale
Prakash D Nallathamby, Xiao-Hong Nancy Xu

Abstract

We have synthesized and purified silver nanoparticles (Ag NPs) (11.3+/-2.3 nm) that are stable (non-aggregated) in cell culture medium and inside single living cells. We have developed new imaging methods to characterize sizes and number of single NPs in the medium and in single living cells in real-time and determine their stability (non-aggregation) in the medium and in single living cells at single NP resolution. These new approaches allow us to study toxic and therapeutic effects of single Ag NPs on tumor cells (L929, mouse fibroblast cells) with determined sizes and concentrations (doses) of NPs over time at single NP and single cell resolution. We found that Ag NPs inhibited the growth and division of tumor cells and their nuclei, in a dose and time dependent manner, showing significant inhibitory effects and abnormal cells with giant undivided nuclei or multiple nuclei beyond 12 h incubation. The results show that Ag NPs inhibited the segregation of chromosomes, but not their replication. Intracellular Ag NPs were well distributed in the cell population, and located in the nuclei and cytoplasm with higher numbers in the cytoplasm. This study demonstrates the possibility of using Ag NPs to inhibit the growth and division ...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H AbkenK Willecke
Aug 30, 1984·Biochemical and Biophysical Research Communications·O Ostling, K J Johanson
Jan 23, 1997·Biochemical and Biophysical Research Communications·M TonettiA De Flora
Sep 25, 1997·International Journal of Cancer. Journal International Du Cancer·R V BlackburnY J Lee
Nov 8, 2002·Journal of Biomedical Optics·Sophia V KyriacouXiao-Hong Nancy Xu
Feb 26, 2003·Cancer Detection and Prevention·Sharon A O'TooleJohn Bonnar
May 7, 2003·Biochemical and Biophysical Research Communications·Xiao-Hong Nancy XuJun Chen
May 28, 2003·Biochemical and Biophysical Research Communications·Xiao-Hong Nancy XuMichelle E Nowak
Nov 5, 2004·Chembiochem : a European Journal of Chemical Biology·Xin GanGenxi Li
Jul 15, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Laura Braydich-StolleMarie-Claude Hofmann
Aug 30, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S M HussainJ J Schlager
Oct 13, 2005·Chemical Communications : Chem Comm·Raymond Wai-Yin SunChi-Ming Che
Apr 14, 2006·Current Drug Delivery·Sandip B Tiwari, Mansoor M Amiji
May 16, 2007·Infection and Immunity·Vanessa C CoimbraMichel Rabinovitch
Aug 21, 2008·Accounts of Chemical Research·Catherine J MurphySarah C Baxter
Feb 25, 2009·ACS Nano·P V AshaRaniSuresh Valiyaveettil
Mar 10, 2009·Methods : a Companion to Methods in Enzymology·Wenjuan LiaoWei-Guo Zhu
Jul 21, 2009·Biochemical Society Transactions·Darragh G McArtGillian R Wasson
Aug 19, 2009·Biotechnology Advances·Ramanathan VaidyanathanSangiliyandi Gurunathan
Oct 20, 2009·Biochemical and Biophysical Research Communications·Nobuhiko Miura, Yasushi Shinohara
Oct 24, 2009·Environmental Science & Technology·Julia FabregaJamie R Lead

❮ Previous
Next ❯

Citations

Jun 15, 2010·Analytical and Bioanalytical Chemistry·Kerry J LeeXiao-Hong Nancy Xu
Mar 22, 2013·Nanomedicine·Shakti Ranjan SatapathyChanakya Nath Kundu
Aug 10, 2013·Nanotoxicology·Matthew C StensbergMaria S Sepúlveda
Jul 1, 2012·Biomolecules & Therapeutics·Kyeongah KangJong-Seok Lim
Jan 28, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T M StępkowskiM Kruszewski
Jul 29, 2011·Nanomedicine·Matthew Charles StensbergMaría Soledad Sepúlveda
May 3, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Zarmina MuhammadNosheen Fatima Rana
Jul 12, 2019·Anti-cancer Agents in Medicinal Chemistry·Tran Q HuyMatteo Tonezzer
Sep 29, 2018·Nanomaterials·Ahmed Abdal DayemSsang-Goo Cho
Nov 26, 2018·Journal of Colloid and Interface Science·Karthikeyan ManivannanJem-Kun Chen
Apr 2, 2021·Nanoscale Advances·Preeyaporn SongkiatisakXiao-Hong Nancy Xu
Sep 22, 2016·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Lauren M BrowningXiao-Hong Nancy Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.