Study of EHS type IV collagen lacking Goodpasture's epitope in glomerulonephritis in rats

Kidney International
W K BoltonB C Sturgill

Abstract

The Goodpasture's epitope has been mapped to the alpha 3 non-collagenous chain (NC1) of type (IV) collagen [alpha 3col(IV)]. We have developed a model of experimental autoimmune glomerulonephritis (EAG) in rats immunized once with collagenase solubilized GBM (csGBM). Engelbreth-Holm-Swarm (EHS) tumor contains abundant col(IV) with little or no alpha 3col(IV). To test the hypothesis that antigens related to Goodpasture epitope are required to produce EAG in our model, we immunized rats once with 40 micrograms csEHS. Positive controls immunized with csGBM developed typical EAG with GBM bound antibody, proteinuria, and glomerulonephritis. EHS rats developed circulating and bound antibody to mesangium and tubular basement membrane with minimal GBM deposits, but did not develop proteinuria or glomerulonephritis. Although circulating antibody in EHS rats bound to csGBM by ELISA, there was no binding in ELISA to M2 antigen containing the Goodpasture epitope while EAG rat's serum did bind. By Western blot with antisera to Goodpasture epitope, EHS antigen was less complex than GBM in the monomer/dimer regions and appeared to lack NC1 corresponding to alpha 3col(IV). Blotting with sera from EHS rats demonstrated reactivity to various com...Continue Reading

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Citations

Nov 25, 2003·Kidney International·Lanlin ChenWarren Kline Bolton
Nov 25, 2003·Seminars in Nephrology·Dorin-Bogdan BorzaBilly G Hudson
Jun 20, 2003·The New England Journal of Medicine·Billy G HudsonEric G Neilson
Jan 26, 2008·Microscopy Research and Technique·Jamshid KhoshnoodiBilly G Hudson
Sep 29, 2006·Journal of the American Society of Nephrology : JASN·Lanlin ChenW Kline Bolton
Aug 28, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marielle A OttenMohamed R Daha
May 29, 2002·The Journal of Laboratory and Clinical Medicine·An-Ming LuoW Kline Bolton
Nov 1, 1996·Kidney International·W K Bolton

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