PMID: 11933045Apr 5, 2002Paper

Study of expression of myelin basic proteins (MBPs) in developing rat brain using a novel antibody reacting with four major isoforms of MBP

Journal of Neuroscience Research
Kyoichi AkiyamaHiroaki Asou

Abstract

Myelin basic proteins (MBPs) are the major protein components of myelin. MBP isoforms are known to have different expression patterns. In order to distinguish the different expression patterns on myelination, we have developed a novel antibody reacting with the four major isoforms of MBPs with molecular masses of 21.5 kDa, 18.5 kDa, 17.0 kDa, and 14.0 kDa. These MBPs were initially separated by acid urea gel and sodium dodecyl sulfate polyacrylamide gel electrophoreses and detected with the luminol reaction. Then the antibody developed was used to determine the relative amounts of MBP isoforms. The MBPs of oligodendrocytes were detected by the enhanced luminol reaction using Renaissance (Dupont NEN, Boston, MA). From the immunological aspect, the MBP monoclonal antibody (Sires et al. [1981] Science 214:87-89) was revealed to recognize MBPs with molecular masses of 21.5 kDa and 18.5 kDa. Furthermore, we found that Ile-166 in the rat 18.5-kDa MBP isomers was replaced by methionine. The 14.0-kDa and 18.5-kDa isoforms of MBP are the most abundant MBP species and comprise more than 70% of the total MBPs in 3.5-and 24-month-old rats. MBPs are expressed during development and the compositions of MBPs in mature (3.5 months old) and age...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E BarbareseJ H Carson
Jul 1, 1974·The Biochemical Journal·P R Dunkley, P R Carnegie
Sep 10, 1995·Archives of Biochemistry and Biophysics·C BouliasM A Moscarello
Apr 29, 1998·Journal of Neuroscience Research·Y SakuraiH Asou
Jul 2, 1999·Experimental Dermatology·K Akiyama, T Senshu

❮ Previous
Next ❯

Citations

Oct 23, 2002·Journal of Neuroscience Research·Ichiro SugiyamaHiroaki Asou
Jul 23, 2008·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·A LehotzkyJ Ovádi
Jul 7, 2011·Journal of Neuroinflammation·Samuel J JacksonDavid Baker
Jan 8, 2004·The Journal of Cell Biology·Jenny HsiehFred H Gage
Jul 15, 2015·Translational Proteomics·Teresa M EvansWilliam E Haskins
Aug 10, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·V M MillerR F Seegal
Aug 9, 2006·Journal of Neurochemistry·Ming Cheng LiuKevin K W Wang
Feb 12, 2009·Journal of Neuroendocrinology·T Kikusui, Y Mori
Sep 8, 2007·The American Journal of Pathology·Yun Ju C SongPoul H Jensen
May 23, 2012·Biochemical and Biophysical Research Communications·Graham S T SmithGeorge Harauz
Jan 18, 2006·Reviews in Medical Virology·Adam M SonabendMaciej S Lesniak
Mar 5, 2016·Endocrinology·Kathleen E MorrisonTracy L Bale
Aug 20, 2003·Development·Carole MathisEmiliana Borrelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.