Study of human cutaneous sensory corpuscles using double immunolabelling and confocal laser scanning microscopy

The Anatomical Record
J A VegaJ M García-Fernández

Abstract

The main constituents of sensory corpuscles, i.e., the central axon, the periaxonic Schwann-related, cells, and the perineurial-related cells, can be identified light microscopically by simple immunohistochemistry using specific antibodies. This paper demonstrates the usefulness of double immunolabelling for light and confocal laser-scanning microscopy (CLSM) in the study of human cutaneous sensory corpuscles. Antibodies directed against neurofilament proteins (NFPs) and S-100 protein were used to label the central axon and the lamellar cells of Meissner corpuscles or the inner-core lamellae of digital cutaneous Pacinian corpuscles, respectively. Samples were obtained from subjects with normal sensitivity and from patients with paresthesia or absence of clinical sensitivity. Single and double immunolabelling was performed, and the sections were studied by light or CLSM microscopy. Double immunolabelling was effective for simultaneous observation of the central axon (NFP-positive) and periaxonic Schwann-related (S-100 protein-positive) cells in sensory corpuscles from normal human digital skin. The images that were obtained with both methods were comparable, but the axonic profiles were sharper with diaminobenzidine (DAB) used a...Continue Reading

References

Mar 1, 1988·Archives of Histology and Cytology·B L Munger, C Ide
Dec 1, 1984·The Anatomical Record·H HachisukaH Nomura
Oct 1, 1995·Cell and Tissue Research·H SannF K Pierau
Jul 1, 1996·Microscopy Research and Technique·J A VegaM E Del Valle

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Citations

Nov 18, 2008·Microscopy Research and Technique·José A VegaJuan M Cobo
Sep 19, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·R CaboO García-Suárez
Jun 3, 2016·IEEE Transactions on Haptics·Trung Quang PhamTakaki Miyata
Jan 20, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D GuinardR Saxod
Oct 24, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ji Hyun KimShunichi Shibata
Sep 18, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J SedýJ Kucera
Jun 28, 2017·Surgical and Radiologic Anatomy : SRA·Komei KobayashiShinichi Abe

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