Aug 1, 1983

Study of in vivo cellular metabolism by phosphorus 31 nuclear magnetic resonance

M BernardP J Cozzone


Phosphorus-31 nuclear magnetic resonance spectroscopy has been recently increasingly used to study cellular metabolism in a manner respecting the cell integrity. Intrinsic advantages of the phosphorus nucleus for in vivo NMR studies are discussed in this review together with some selected applications. A particular emphasis is layed on metabolite identification and quantitation (relative and absolute concentrations), the measurement of intracellular pH and the problem of cellular compartmentation. The determination of metabolite fluxes under normal and abnormal biological and physiological conditions, and the in vivo direct measurement by saturation transfer techniques of kinetic parameters for enzymatic reactions at equilibrium, are illustrated by several examples taken from the available literature and work carried out in this laboratory. Whenever possible, and appropriate, the NMR approach has been compared with other more classical techniques of investigation. The future and the potentialities of phosphorus-31 NMR study of intact biological systems, the clinical applications and the foreseeable interfacing with imaging techniques are evaluated. The concept of "functional imaging" versus "anatomic imaging" is proposed to ill...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
In Vivo NMR Spectroscopy
Magnetic Resonance Imaging
Phosphate Measurement
Cell Nucleus
Red Phosphorus
Phosphorus Measurement
Imaging Techniques

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