Study of intestinal cell differentiation with monoclonal antibodies to intestinal cell surface components

Developmental Biology
A Quaroni, K J Isselbacher

Abstract

Monoclonal antibodies that react with antigens of the plasma membrane of rat intestinal villus and crypt cells have been prepared by fusion of mouse myeloma (NSI) cells with spleen cells of mice immunized with various intestinal cellular fractions, including the luminal membrane of adult villus and crypt cells, and of newborn rat intestinal cells. The antigenic targets of most antibodies have been identified. They include major protein components of the brush border (luminal) membrane of adult villus cells (sucrase-isomaltase, maltase, lactase, aminopeptidase N, alkaline phosphatase) and newly identified protein antigens specific for intestinal epithelial cells. Of 25 independently derived monoclonal antibodies prepared, 18 reacted exclusively with the brush border membrane of the villus cells, confirming its unique protein composition. Antibodies specifically staining the crypt cells, the newly differentiated epithelial cells present in the lower half of the villi, the top villus cells, and both villus and crypt cells were also obtained and characterized. These antibodies have been used to study the expression of cell- and tissue-specific functions during differentiation and development of the intestinal epithelium. Contrary t...Continue Reading

References

Feb 1, 1979·The Biochemical Journal·P R Flanagan, G G Forstner
Jan 1, 1979·Journal of Nutritional Science and Vitaminology·K YamadaN Hosoya
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K Radke, G S Martin
Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
May 1, 1985·The Journal of Cell Biology·A Quaroni
Jan 1, 1968·The Biochemical Journal·G G ForstnerK J Isselbacher
Feb 28, 1972·Biochimica Et Biophysica Acta·S Schlegel-HaueterG Semenza
Oct 1, 1972·The Journal of Clinical Investigation·D H Alpers
Nov 1, 1971·Experimental Cell Research·I Macpherson, A Bryden
Oct 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G DOELLN KRETCHMER
Dec 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C NordströmL Josefsson
Sep 1, 1965·Experimental Cell Research·A B CairnieG G Steel
Mar 1, 1966·Analytical Biochemistry·M Messer, A Dahlqvist
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H P HauriK J Isselbacher
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bjerknes, H Cheng
Jan 1, 1972·FEBS Letters·H Glossmann, D M. Neville

Citations

Sep 15, 1986·Experientia·C Rochette-EglyK Haffen
Mar 1, 1996·In Vitro Cellular & Developmental Biology. Animal·A AmetaniK Hashimoto
Oct 7, 2005·Histochemistry and Cell Biology·Adrian R WestPhillip S Oates
Jan 1, 1995·Biology of the Cell·J N FreundG Morel
Mar 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·S Polak-CharconY Ben-Shaul
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A Quaroni
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W Hong, D Doyle
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·H KomanoS Itohara
Aug 1, 1987·The Journal of Cell Biology·J M WilsonM R Neutra
Mar 3, 1998·Journal of Pediatric Gastroenterology and Nutrition·E H Van BeersHans A Büller
Jul 21, 1998·Journal of Pediatric Gastroenterology and Nutrition·E H Van BeersHans A Büller
Dec 1, 1996·Alcoholism, Clinical and Experimental Research·G EstradaM D López-Tejero
Sep 3, 1998·Alcoholism, Clinical and Experimental Research·G EstradaM D López-Tejero
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·E H Van BeersJ Dekker
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Melissa VerburgJan Dekker
Nov 30, 1992·FEBS Letters·M RossiS Auricchio
Jul 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bruno StiegerL Landmann
May 6, 2005·American Journal of Physiology. Cell Physiology·Franca Golin-BiselloNadia A Ameen
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adrian R West, Phillip S Oates
Jan 17, 2003·American Journal of Physiology. Cell Physiology·Nadia A AmeenPedro J I Salas
Nov 23, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·M FijlstraW J E Tissing
Jul 1, 1996·Journal of Pediatric Gastroenterology and Nutrition·E FerrettiA Moore
Apr 20, 2002·The Journal of Biological Chemistry·Céline LemmersAndré Le Bivic
May 28, 1992·International Journal of Cancer. Journal International Du Cancer·A QuaroniS R Hamilton
Sep 26, 2000·American Journal of Physiology. Cell Physiology·A QuaroniC Ap Rhys
Feb 29, 2000·Molecular Pharmacology·J MadonBruno Stieger
Feb 1, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·N Nanda NanthakumarW Allan Walker
Apr 2, 2002·The Journal of Nutrition·Carla Thomas, Phillip S Oates
Nov 27, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pascal FreiBruno Stieger

Related Concepts

Alkaline Phosphatase
Lysosomal alpha-Glucosidase
Aminopeptidase
Metazoa
Monoclonal Antibodies
Lactrase
Cell Differentiation Process
Plasma Membrane
SDS-PAGE
Immunofluorescence Assay

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